FAS ELISA 试剂盒
(1 reference)-
- 抗原 See all FAS ELISA试剂盒
- FAS (TNF Receptor Superfamily, Member 6 (FAS))
- 抗原表位
- AA 17-173
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Soluble FAS
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: R17-N173 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <3pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: R17-N173 - Featured
- Discover our best selling FAS ELISA Kit
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- Discover our top product FAS ELISA Kit
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TNF Receptor Superfamily, Member 6 (FAS) ELISA Kit
FAS 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
TNF Receptor Superfamily, Member 6 (FAS) ELISA KitFAS 适用: 人 Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
TNF Receptor Superfamily, Member 6 (FAS) ELISA KitFAS 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
TNF Receptor Superfamily, Member 6 (FAS) ELISA KitFAS 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
TNF Receptor Superfamily, Member 6 (FAS) ELISA KitFAS 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
TNF Receptor Superfamily, Member 6 (FAS) ELISA KitFAS 适用: 小鼠 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
TNF Receptor Superfamily, Member 6 (FAS) ELISA KitFAS 适用: 小鼠 AA 22-169 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
TNF Receptor Superfamily, Member 6 (FAS) ELISA KitFAS 适用: 大鼠 Colorimetric Sandwich ELISA 40-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
TNF Receptor Superfamily, Member 6 (FAS) ELISA KitFAS 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
TNF Receptor Superfamily, Member 6 (FAS) ELISA KitFAS 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Contains 1 death domain.
Tissue Specificity: Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. .
- 板类型
- Pre-coated
- 实验流程
- human FAS ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FAS has been precoated onto 96-well plates. Standards(NSO, R17-N173) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FAS is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FAS amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human FAS standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human FAS standard solution and each sample is measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 138, Standard deviation: 5.80, CV(%): 4.2
- Sample 2: n=16, Mean(pg/ml): 828, Standard deviation: 25.67, CV(%): 3.1
- Sample 3: n=16, Mean(pg/ml): 1507, Standard deviation: 70.83, CV(%): 4.7,
- Sample 1: n=24, Mean(pg/ml): 146, Standard deviation: 10.95, CV(%): 7.5
- Sample 2: n=24, Mean(pg/ml): 839, Standard deviation: 45.31, CV(%): 5.4
- Sample 3: n=24, Mean(pg/ml): 1512, Standard deviation: 83.16, CV(%): 5.5
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury." in: World journal of gastroenterology, Vol. 19, Issue 4, pp. 492-502, (2013) (PubMed).
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: "Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury." in: World journal of gastroenterology, Vol. 19, Issue 4, pp. 492-502, (2013) (PubMed).
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- 抗原 See all FAS ELISA试剂盒
- FAS (TNF Receptor Superfamily, Member 6 (FAS))
- 别名
- FAS (FAS 产品)
- 别名
- ALPS1A ELISA Kit, APO-1 ELISA Kit, APT1 ELISA Kit, CD95 ELISA Kit, FAS1 ELISA Kit, FASTM ELISA Kit, TNFRSF6 ELISA Kit, AI196731 ELISA Kit, APO1 ELISA Kit, TNFR6 ELISA Kit, Tnfrsf6 ELISA Kit, lpr ELISA Kit, Fas cell surface death receptor ELISA Kit, Fas (TNF receptor superfamily member 6) ELISA Kit, FAS ELISA Kit, Fas ELISA Kit, fas ELISA Kit
- 背景
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Protein Function: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). .
Background: Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor(NGF)/tumour necrosis factor(TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48- kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo. The FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis.
Synonyms: Tumor necrosis factor receptor superfamily member 6,Apo-1 antigen,Apoptosis-mediating surface antigen FAS,FASLG receptor,CD95,FAS,APT1, FAS1, TNFRSF6,
Full Gene Name: Tumor necrosis factor receptor superfamily member 6
Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein. - 基因ID
- 355
- UniProt
- P25445
- 途径
- p53 Pathway, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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