Endothelin 1 ELISA 试剂盒
(2 references)-
- 抗原 See all Endothelin 1 (EDN1) ELISA试剂盒
- Endothelin 1 (EDN1)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 10-1000 pg/mL
- 最低检测浓度
- 10 pg/mL
- 应用范围
- ELISA
- 原理
- Human Endothelin-1 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Human Endothelin-1 (EDN1)
- 灵敏度
- 10 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling EDN1 ELISA Kit
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Endothelin 1 (EDN1) ELISA Kit
EDN1 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 pg/mL - 50 pg/mL Cell Culture Supernatant, Plasma, Serum
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 人 Colorimetric Sandwich ELISA 1.25 pg/mL - 80 pg/mL Cell Culture Supernatant, Plasma, Serum
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 人 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 小鼠 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 人 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Saliva, Serum, Urine
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 大鼠 Colorimetric Sandwich ELISA 1.25 pg/mL - 80 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 大鼠 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 豚鼠 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 适用: Pig Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 兔 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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: "Effect of minimized perfusion circuit on brain injury markers carnosinase and brain-type fatty binding protein in coronary artery bypass grafting patients." in: Artificial organs, Vol. 37, Issue 2, pp. 128-35, (2013) (PubMed).
: "Tumor endothelin-1 enhances metastatic colonization of the lung in mouse xenograft models of bladder cancer." in: The Journal of clinical investigation, Vol. 121, Issue 1, pp. 132-47, (2011) (PubMed).
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: "Effect of minimized perfusion circuit on brain injury markers carnosinase and brain-type fatty binding protein in coronary artery bypass grafting patients." in: Artificial organs, Vol. 37, Issue 2, pp. 128-35, (2013) (PubMed).
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- 抗原 See all Endothelin 1 (EDN1) ELISA试剂盒
- Endothelin 1 (EDN1)
- 别名
- Endothelin-1 (EDN1 产品)
- 别名
- et1 ELISA Kit, et-1 ELISA Kit, Edn1 ELISA Kit, EDN ELISA Kit, ET-1 ELISA Kit, ET1 ELISA Kit, PPET1 ELISA Kit, HDLCQ7 ELISA Kit, preproET ELISA Kit, Et1 ELISA Kit, fb14d01 ELISA Kit, wu:fb14d01 ELISA Kit, PPET-1 ELISA Kit, EDN1 ELISA Kit, edn1 ELISA Kit, edn1-a ELISA Kit, edn1-b ELISA Kit, endothelin 1 ELISA Kit, endothelin 1 S homeolog ELISA Kit, endothelin 1 L homeolog ELISA Kit, edn1 ELISA Kit, EDN1 ELISA Kit, Edn1 ELISA Kit, edn1.S ELISA Kit, edn1.L ELISA Kit
- 背景
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Gene Names: EDN1
Protein names: Endothelin-1 (Preproendothelin-1) (PPET1) [Cleaved into: Endothelin-1 (ET-1), Big endothelin-1]
- 基因ID
- 1906
- UniProt
- P05305
- 途径
- Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size
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