PTGS2 ELISA 试剂盒
(2 references)-
- 抗原 See all PTGS2 ELISA试剂盒
- PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.2-300 ng/mL
- 最低检测浓度
- 1.2 ng/mL
- 应用范围
- ELISA
- 原理
- Human COX-2 (PTGS2) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects human COX-2. Other species not determined.
- 灵敏度
- 1.2 ng/mL
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling PTGS2 ELISA Kit
- Top Product
- Discover our top product PTGS2 ELISA Kit
-
Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) ELISA Kit
PTGS2 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) ELISA KitPTGS2 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) ELISA KitPTGS2 适用: 大鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma, Serum
Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) ELISA KitPTGS2 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) ELISA KitPTGS2 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) ELISA KitPTGS2 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) ELISA KitPTGS2 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) ELISA KitPTGS2 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) ELISA KitPTGS2 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2) ELISA KitPTGS2 适用: 犬 Colorimetric Competition ELISA 0.5-10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
-
- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
-
: "Cyclooxygenase 2 (rs2745557) Polymorphism and the Susceptibility to Benign Prostate Hyperplasia and Prostate Cancer in Egyptians." in: Biochemical genetics, Vol. 54, Issue 3, pp. 326-36, (2017) (PubMed).
: "Tumor endothelin-1 enhances metastatic colonization of the lung in mouse xenograft models of bladder cancer." in: The Journal of clinical investigation, Vol. 121, Issue 1, pp. 132-47, (2011) (PubMed).
-
: "Cyclooxygenase 2 (rs2745557) Polymorphism and the Susceptibility to Benign Prostate Hyperplasia and Prostate Cancer in Egyptians." in: Biochemical genetics, Vol. 54, Issue 3, pp. 326-36, (2017) (PubMed).
-
- 抗原 See all PTGS2 ELISA试剂盒
- PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))
- 别名
- COX-2 (PTGS2 产品)
- 别名
- COX-2 ELISA Kit, COX2 ELISA Kit, GRIPGHS ELISA Kit, PGG/HS ELISA Kit, PGHS-2 ELISA Kit, PHS-2 ELISA Kit, hCox-2 ELISA Kit, Cox-2 ELISA Kit, Pghs2 ELISA Kit, TIS10 ELISA Kit, Cox2 ELISA Kit, cox-2 ELISA Kit, CEF147 ELISA Kit, PGHS2 ELISA Kit, PHSII ELISA Kit, cox2 ELISA Kit, gripghs ELISA Kit, pgg/hs ELISA Kit, pghs-2 ELISA Kit, phs-2 ELISA Kit, PTGS2 ELISA Kit, hcox-2 ELISA Kit, fj02a10 ELISA Kit, ptgs2 ELISA Kit, unp1239 ELISA Kit, wu:fj02a10 ELISA Kit, zCOX-2 ELISA Kit, si:dkey-97o5.6 ELISA Kit, prostaglandin-endoperoxide synthase 2 ELISA Kit, prostaglandin G/H synthase 2 ELISA Kit, prostaglandin-endoperoxide synthase 2S homeolog ELISA Kit, prostaglandin-endoperoxide synthase 2a ELISA Kit, prostaglandin-endoperoxide synthase 2b ELISA Kit, PTGS2 ELISA Kit, Ptgs2 ELISA Kit, LOC100136456 ELISA Kit, ptgs2.S ELISA Kit, ptgs2 ELISA Kit, ptgs2a ELISA Kit, ptgs2b ELISA Kit
- 背景
-
Gene Names: PTGS2 COX2
Protein names: Prostaglandin G/H synthase 2 (EC 1.14.99.1) (Cyclooxygenase-2) (COX-2) (PHS II) (Prostaglandin H2 synthase 2) (PGH synthase 2) (PGHS-2) (Prostaglandin-endoperoxide synthase 2)
- 基因ID
- 5743
- UniProt
- P35354
- 途径
- Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
-
