APOC3 ELISA 试剂盒
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- 抗原 See all APOC3 ELISA试剂盒
- APOC3 (Apolipoprotein C-III (APOC3))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 2.5-10.000 pg/mL
- 最低检测浓度
- 2.5 pg/mL
- 应用范围
- ELISA
- 原理
- Human ApoC3 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Alpha-2 Microglobulin, ApoA, ApoC1, ApoC2, ApoE, ApoH, Artemin, BMP-6, Calbindin, CK beta 8-1 (CCL23), CNTF, EMMPRIN (CD147), Endostatin, Fibrinogen, FLRG, TNFSF18 (GITR Ligand), GPBB, Hemoglobin, HTRA2, IL-17E, IL-1 R3 (IL1 R Acp), Lactoferrin, Mesothelin, PAPP-A, PD-ECGF (Thymidine Phosphorylase), PDGF R alpha, Pepsinogen 2, Periostin, Persephin, PTH, RAP, SAA (Serum Amyloid A), Serpin F1 (PEDF), Tie-1, TPA, Thrombospondin 2, Ubiquitin+1, Vitronectin, VWF
- 灵敏度
- 2.5 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling APOC3 ELISA Kit
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- Discover our top product APOC3 ELISA Kit
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Apolipoprotein C-III (APOC3) ELISA Kit
APOC3 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma
Apolipoprotein C-III (APOC3) ELISA KitAPOC3 适用: 人 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Apolipoprotein C-III (APOC3) ELISA KitAPOC3 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Apolipoprotein C-III (APOC3) ELISA KitAPOC3 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein C-III (APOC3) ELISA KitAPOC3 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Plasma, Serum
Apolipoprotein C-III (APOC3) ELISA KitAPOC3 适用: 猴 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein C-III (APOC3) ELISA KitAPOC3 适用: 大鼠 Colorimetric Competition ELISA 31.25 ng/mL - 2000 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein C-III (APOC3) ELISA KitAPOC3 适用: 人 Colorimetric Competition ELISA 15.6 ng/mL - 1000 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein C-III (APOC3) ELISA KitAPOC3 适用: 小鼠 Colorimetric Competition ELISA 18.75 ng/mL - 1200 ng/mL Plasma, Serum, Tissue Homogenate
Apolipoprotein C-III (APOC3) ELISA KitAPOC3 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples500,000 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 样品制备
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For example, add 2 μL of serum/plasma into a tube with 998.0 μL Assay Diluent to prepare a 500-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 500-fold diluted sample into a tube with 1998 μL Assay Diluent to prepare a final 500,000 fold diluted sample.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all APOC3 ELISA试剂盒
- APOC3 (Apolipoprotein C-III (APOC3))
- 别名
- ApoC3 (APOC3 产品)
- 别名
- APOC3 ELISA Kit, APOCIII ELISA Kit, HALP2 ELISA Kit, Apoc ELISA Kit, ApoC-III ELISA Kit, apo-CIII ELISA Kit, Apo-CIII ELISA Kit, apolipoprotein C3 ELISA Kit, apolipoprotein C-III ELISA Kit, APOC3 ELISA Kit, Apoc3 ELISA Kit
- 背景
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Gene Names: APOC3
Protein names: Apolipoprotein C-III (Apo-CIII) (ApoC-III) (Apolipoprotein C3)
- 基因ID
- 345
- UniProt
- P02656
- 途径
- Carbohydrate Homeostasis, Lipid Metabolism
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