alpha 2 Macroglobulin ELISA 试剂盒
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- 抗原 See all alpha 2 Macroglobulin (A2M) ELISA试剂盒
- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 4-3.000 ng/mL
- 最低检测浓度
- 4 ng/mL
- 应用范围
- ELISA
- 原理
- Human Alpha-2 Macroglobulin ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects human Alpha 2-M. Other species not determined.
- 灵敏度
- 4 ng/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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alpha-2-Macroglobulin (A2M) ELISA Kit
A2M 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Urine
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 小鼠 Colorimetric Competition ELISA 3.7 ng/mL - 300 ng/mL Plasma, Serum, Urine
alpha-2-Macroglobulin (A2M) ELISA KitHigh Sensitivity A2M 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 大鼠 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Plasma, Serum, Urine
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 人 Colorimetric Competition ELISA 1.25 μg/mL - 20 μg/mL Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 人 Colorimetric Sandwich ELISA 62.5 ng/mL - 4000 ng/mL Cell Culture Supernatant, Plasma, Serum
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 人 Colorimetric Sandwich ELISA 78.125 ng/mL - 5000 ng/mL Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 大鼠 Colorimetric Sandwich ELISA 0.469 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: Cow Colorimetric Sandwich ELISA 0.156 μg/mL - 10 μg/mL Plasma, Serum, Tissue Homogenate
alpha-2-Macroglobulin (A2M) ELISA KitA2M 适用: 兔 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples2,000 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all alpha 2 Macroglobulin (A2M) ELISA试剂盒
- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
- 别名
- alpha 2 M (A2M 产品)
- 别名
- A2MD ELISA Kit, CPAMD5 ELISA Kit, FWP007 ELISA Kit, S863-7 ELISA Kit, A2MAC1 ELISA Kit, A2m1 ELISA Kit, A2maa ELISA Kit, Mam ELISA Kit, A2mp ELISA Kit, A2M ELISA Kit, LOC733429 ELISA Kit, endod ELISA Kit, cpamd5 ELISA Kit, fwp007 ELISA Kit, s863-7 ELISA Kit, a2mb ELISA Kit, endodermin ELISA Kit, Alpha-2-M ELISA Kit, alpha-2-macroglobulin ELISA Kit, pregnancy-zone protein L homeolog ELISA Kit, alpha-2-macroglobulin S homeolog ELISA Kit, pregnancy zone protein ELISA Kit, alpha-2-macroglobulin-like ELISA Kit, A2M ELISA Kit, A2m ELISA Kit, LOC477699 ELISA Kit, pzp.L ELISA Kit, AZL_c00450 ELISA Kit, LOC100349077 ELISA Kit, a2m ELISA Kit, LOC100061656 ELISA Kit, LOC100090399 ELISA Kit, a2m.S ELISA Kit, LOC100469973 ELISA Kit, LOC100595735 ELISA Kit, PZP ELISA Kit, LOC100353095 ELISA Kit, LOC101122940 ELISA Kit, LOC101801552 ELISA Kit, LOC100911545 ELISA Kit
- 背景
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Gene Names: A2M CPAMD5 FWP007
Protein names: Alpha-2-macroglobulin (Alpha-2-M) (C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5)
- 基因ID
- 2
- UniProt
- P01023
- 途径
- Lipid Metabolism
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