FASL ELISA 试剂盒
-
- 抗原 See all FASL ELISA试剂盒
- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.63-1000 pg/mL
- 最低检测浓度
- 15.63 pg/mL
- 应用范围
- ELISA
- 原理
- This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.
- 样品类型
- Serum, Plasma, Cell Culture Supernatant
- Analytical Method
- Quantitative
- 特异性
- Fas Ligand, Human
- 灵敏度
- 3.42 pg/mL
- 组件
- Plate, Standard, Diluent
- 试剂未包括
-
Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution. - Featured
- Discover our best selling FASL ELISA Kit
- Top Product
- Discover our top product FASL ELISA Kit
-
Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA Kit
FASL 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA KitFASL 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA KitFASL 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA KitFASL 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA KitFASL 适用: 人 AA 134-281 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA KitFASL 适用: 小鼠 AA 132-279 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA KitFASL 适用: 大鼠 AA 127-278 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA KitFASL 适用: 大鼠 Colorimetric Sandwich ELISA 90-60000 pg/mL Cell Culture Supernatant, Plasma, Serum
-
- 样本量
- 100 μL
- 实验时间
- 3 - 4 h
- 板类型
- Pre-coated
- 限制
- 仅限研究用
-
- 缓冲液
- Buffer contains: 0.02 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
-
Intended for research use only and are not for use in diagnostic or therapeutic procedures.
Treat all chemicals with caution because they can be potentially hazardous.
It is recommended that this product is handled only by persons who have been trained in laboratory techniques and in accordance with the principles of good laboratory practice. Wear personal protection equipment such as laboratory coat, safety glasses and gloves.
Avoid direct contact with skin or eyes. Wash immediately with water in the case of contact with skin or eyes. Avoid contact of skin or mucous membranes with kit reagents or specimens. See material safety data sheet(s) for specific advice.
Pure water or deionized water must be used for reagent preparation.
The Stop Solution provided with this kit is an acid solution. Wear personal protection equipment with caution.
Do not expose kit reagents to strong light during storage and incubation.
Rubber or disposable latex gloves should be worn while handling kit reagents or specimens.
Avoid contact of substrate solution with oxidizing agents and metal.
Avoid splashing or generation of aerosols.
Use disposable pipette tips and/or pipettes to avoid microbial or cross-contamination of reagents or specimens which may invalidate the test.
Use clean, dedicated reagent trays for dispensing the conjugate and substrate reagent.
Exposure to acid inactivates the HRP and antibody conjugate.
Substrate solution must be warmed to room temperature prior to use.
Decontaminate and dispose specimens and all potentially contaminated materials as they could contain infectious agents. The preferred method of decontamination is autoclaving for a minimum of 1 hour at 121.5 °C.
Liquid wastes not containing acid and neutralized waste may be mixed with sodium hypochlorite in volumes such that the final mixture contains 1.0 % sodium hypochlorite. Allow 30 minutes for effective decontamination. Liquid waste containing acid must be neutralized prior to the addition of sodium hypochlorite.
All reagents including microplate, samples, standards and working solution should be warmed to room temperature before use.
To obtain accurate results, using adhesive film to seal the plate during incubation is suggested.
It is recommended that all samples and standards be assayed in duplicate.
Avoid foaming when mixing or reconstituting solutions containing protein.
To avoid cross-contamination, use separate reservoirs for each reagent and change pipette tips between each standard, sample and reagent.
When using an automated plate washer, adding a 30 seconds soak period before washing step and/or rotating the plate between wash steps may improve assay precision.
When pipetting reagents, maintain a consistent order of addition from well-to-well.
Keep Substrate solution protected from direct strong light. Substrate Solution should turn to gradations of blue after a proper color development.
Read absorbance within 30 minutes after adding stop solution.
Take care not to scratch the inner surface of the microwells. - 储存条件
- 4 °C
- 储存方法
-
Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit
-
- 抗原 See all FASL ELISA试剂盒
- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
- 别名
- FASLG (FASL 产品)
- 别名
- ALPS1B ELISA Kit, APT1LG1 ELISA Kit, APTL ELISA Kit, CD178 ELISA Kit, CD95-L ELISA Kit, CD95L ELISA Kit, FASL ELISA Kit, TNFSF6 ELISA Kit, fasl ELISA Kit, Fas-L ELISA Kit, Faslg ELISA Kit, Tnfsf6 ELISA Kit, gld ELISA Kit, Apt1Lg1 ELISA Kit, Fasl ELISA Kit, FASLG ELISA Kit, zgc:162027 ELISA Kit, Fas ligand ELISA Kit, Fas ligand L homeolog ELISA Kit, Fas ligand (TNF superfamily, member 6) ELISA Kit, FASLG ELISA Kit, faslg.L ELISA Kit, Fasl ELISA Kit, Faslg ELISA Kit, faslg ELISA Kit
- 背景
- Fas ligand (also known as TNFSF6, CD178, FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand is a homotrimeric type II transmembrane protein expressed on cytotoxic T lymphocytes. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7. Apoptosis triggered by Fas-Fas ligand binding plays a fundamental role in the regulation of the immune system. Its functions include:T-cell homeostasis, cytotoxic T-cell activity, immune privilege, maternal tolerance, tumor counterattack. Defective Fas-mediated apoptosis may lead to oncogenesis as well as drug resistance in existing tumors. Germline mutation of Fas is associated with autoimmune lymphoproliferative syndrome (ALPS), a childhood disorder of apoptosis.
- 基因ID
- 356
- NCBI登录号
- NM_000639
- UniProt
- P48023
- 途径
- Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
-
