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Thyroglobulin ELISA 试剂盒

TG 适用: 人 Colorimetric Serum
产品编号 ABIN2648834
发货至: 中国
  • 抗原 See all Thyroglobulin (TG) ELISA试剂盒
    Thyroglobulin (TG)
    适用
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    检测方法
    Colorimetric
    应用范围
    ELISA
    样品类型
    Serum
    特异性
    Ultrasensitive ELISA
    灵敏度
    0.03 ng/mL
    产品特性
    Biochemically, Tg is to be understood as a rather complex family of molecules. It is micro heterogeneous with inter- and intra-individual variations (iodination degree, carbohydrate contents etc.). Dimers and several fragments also exist. Additional heterogeneity is due to malignant de-differentiation. Specifically and unspecifically interfering factors in individual sera cause further problems. Therefore, the Tg determination still represents a rather ambitious method.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    样本量
    25 μL
    板类型
    Pre-coated
    实验流程
    Tg of serum samples, controls and calibrators react with the solid phase bound anti-hTg antibody during the first incubation period of 2.5 hours at room temperature. After removal of the non-bound components by a washing step anti-hTg HRP conjugate interacts with the Tg molecules trapped by the immobilized anti-hTg antibody during the second incubation at room temperature over night (> 15 hours). The sandwich type com-plexes formed are separated from unbound material by a further washing step. HRP converts the colorless substrate solution of 3,3',5,5'-tetramethylbenzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 15 min, turning the solution from blue to yellow.
    限制
    仅限研究用
  • 储存条件
    4 °C
  • 抗原 See all Thyroglobulin (TG) ELISA试剂盒
    Thyroglobulin (TG)
    别名
    Tg (Thyroglobulin) (TG 产品)
    别名
    TG ELISA Kit, cb717 ELISA Kit, AITD3 ELISA Kit, TGN ELISA Kit, Tgn ELISA Kit, cog ELISA Kit, thyroglobulin ELISA Kit, TG ELISA Kit, tg ELISA Kit, LOC701526 ELISA Kit, LOC701756 ELISA Kit, LOC100434297 ELISA Kit, Tg ELISA Kit
    途径
    Thyroid Hormone Synthesis
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