2-ThioBarbituric Acid Reactive Substances (TBARS) ELISA 试剂盒
适用: 化学剂
Colorimetric
Plasma, Serum, Urine
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- 抗原
- 2-ThioBarbituric Acid Reactive Substances (TBARS)
- 适用
- 化学剂
- 检测方法
- Colorimetric
- 应用范围
- ELISA
- 样品类型
- Serum, Plasma, Urine
- 灵敏度
- 0.5 μM
- 产品特性
- 2-ThioBarbituric Acid Reactive Substances (TBARS) are naturally present in biological specimens and include lipid hydroperoxides and aldehydes which increase in concentration as a response to oxidative stress.1,2 TBARS assay values are usually reported in malonaldehyde (malondialdehyde, MDA) equivalents, a compound that results from the decomposition of polyunsaturated fatty acid lipid peroxides. The TBARS assay is a well-recognized, established method for quantifying these lipid peroxides, although it has been criticized for its reactivity towards other compounds other than MDA.3 This TBARS Cuvette Assay Kit offers the researcher a straightforward, reproducible and consistent method for analyzing urine for lipid peroxidation products.
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样本量
- 100 μL
- 实验时间
- 0.5 h
- 板类型
- Pre-coated
- 实验流程
- This TBARS Cuvette Assay Kit is based on the reaction of a chromogenic reagent, 2-thiobarbituric acid, with MDA at 25 °C. One molecule of MDA reacts with 2 Molecules of 2-thiobarbituric acid via a Knoevenagel-type condensation to yield a chromophore with absorbance maximum at 532 nm,
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原
- 2-ThioBarbituric Acid Reactive Substances (TBARS)
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