Anti-Gliadin IgA ELISA 试剂盒
适用: 人
Colorimetric
Fecal
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- 抗原
- Anti-Gliadin IgA
- 适用
- 人
- 检测方法
- Colorimetric
- 应用范围
- ELISA
- 原理
- Anti-Gliadin sIgA / IgA ELISA kit allows for an easy, rapid and precise quantitative determination of the secretory IgA and IgA gliadin antibodies in stool. The kit includes all reagents ready to use for preparation of the samples.
- 样品类型
- Fecal
- Analytical Method
- Quantitative
- 灵敏度
- 1.3 mU/g
- 产品特性
- Celiac disease is a chronic illness of the small intestinal mucous membrane. It is caused by an intolerance against gluten, which is found in many cereals. The intake of gluten-containing food leads to inflammation of the small intestinal mucous membrane. The resorption of nutrients is therefore reduced. The symptoms of the disease are reduction of weight, diarrhoea, vomiting, anorexia and tiredness. The growth in children is reduced. The only therapeutic treatment is a gluten-free diet. An untreated celiac disease has been known to increase the risk of non-Hodgkin-lymphoma and colon cancer. In five to ten percent of the patients, celiac disease is associated with diabetes mellitus type 1. Women are more often affected than men. The outcome of the disease is pronounced during infancy and in an age between 30 and 40 years.
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Anti-Gliadin IgA ELISA Kit
适用: 人 Colorimetric Competition ELISA 1-200 U/mL Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样本量
- 100 mg
- 实验时间
- 2 h
- 实验流程
- The Anti-Gliadin sIgA / IgA ELISA kit determines human anti-gliadin sIgA / IgA antibodies according to the "sandwich"-principle. Anti-gliadin antibodies in sample, standard and controls bind to gliadin, which is coated to the microtiter plate. After a washing step a peroxidase labeled detection antibody is added. A second washing step is followed by the addition of the substrate which is converted to a colored product by the peroxidase. The reaction is terminated by the addition of an acidic stop solution. The optical densities are read at 450 nm in a microtiter plate reader. The anti-gliadin concentration can be calculated from the standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原
- Anti-Gliadin IgA
- 别名
- Anti-Gliadin sIgA / IgA
- 物质类
- Antibody
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