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G-CSF ELISA 试剂盒

CSF3 适用: 人 Colorimetric Cell Culture Cells, Plasma, Serum
产品编号 ABIN2648562
发货至: 中国
  • 抗原 See all G-CSF (CSF3) ELISA试剂盒
    G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
    适用
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    检测方法
    Colorimetric
    应用范围
    ELISA
    原理
    Human Granulocyte Colony Stimulating Factor (G-CSF) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Granulocyte Colony Stimulating Factor (G-CSF) concentrations in cell culture supernates, serum, and plasma.
    样品类型
    Serum, Plasma, Cell Culture Cells
    Analytical Method
    Quantitative
    灵敏度
    4 pg/ml
    产品特性
    Human Granulocyte Colony Stimulating Factor (G-CSF) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    样本量
    100 μL
    实验时间
    3.5 h
    板类型
    Pre-coated
    实验流程
    Human Granulocyte Colony Stimulating Factor (G-CSF) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for G-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any G-CSF present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for G-CSF is added to the wells and binds to the combination of capture antibody- G-CSF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of G-CSF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven G-CSF standard dilutions and G-CSF sample concentration determined.
    限制
    仅限研究用
  • 储存条件
    4 °C
  • 抗原 See all G-CSF (CSF3) ELISA试剂盒
    G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
    别名
    G-CSF (CSF3 产品)
    别名
    CSF3 ELISA Kit, G-CSF ELISA Kit, gcsf ELISA Kit, Csfg ELISA Kit, MGI-IG ELISA Kit, C17orf33 ELISA Kit, CSF3OS ELISA Kit, GCSF ELISA Kit, Gcsf ELISA Kit, colony stimulating factor 3 ELISA Kit, colony stimulating factor 3 (granulocyte) a ELISA Kit, colony stimulating factor 3 (granulocyte) ELISA Kit, CSF3 ELISA Kit, csf3a ELISA Kit, Csf3 ELISA Kit
    途径
    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
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