GM-CSF Receptor alpha ELISA 试剂盒
适用: 人
Colorimetric
Cell Culture Cells, Plasma, Serum
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- 抗原
- GM-CSF Receptor alpha
- 适用
- 人
- 检测方法
- Colorimetric
- 应用范围
- ELISA
- 原理
- Human soluble GM-CSF receptor α chain (CD116) ELISA is to be used for the in-vitro quantitative determination of CD116 in serum, plasma, or cell culture supernatants. The assay will recognize both natural and recombinant CD116.
- 样品类型
- Serum, Plasma, Cell Culture Cells
- Analytical Method
- Quantitative
- 灵敏度
- 16 pg/ml
- 产品特性
- Human CD116 ELISA Assay kit is for Research Use Only.
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样本量
- 100 μL
- 实验时间
- 3 - 4 h
- 板类型
- Pre-coated
- 实验流程
- The CD116 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD116 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD116 concentrations and unknowns are pipetted into these wells. During the first incubation, the CD116 antigen and a biotinylated monoclonal antibody specific for CD116 are simultaneously incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD116 present in the samples.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原
- GM-CSF Receptor alpha
- 别名
- CD116 / GM-CSF R
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