AChR Ab ELISA 试剂盒
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- 抗原 See all AChR Ab products
- AChR Ab (Acetylcholine Receptor Antibody (AChR Ab))
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适用
- 人
- 检测方法
- Colorimetric
- 应用范围
- ELISA
- 原理
- Anti-AChR (Acetylcholine Receptor) ELISA Assay kit is intended for the quantitative determination of Anti-AChR in serum by enzyme linked immunoassay (ELISA).
- 样品类型
- Serum
- Analytical Method
- Quantitative
- 灵敏度
- 0.23 nmol/l
- 产品特性
- The Anti-AChR ELISA Assay kit. Autoantibodies to the acetylcholine receptor (AChR) are responsible for failure of the neuromuscular junction in myasthenia gravis, a neuromuscular disease leading to fluctuating muscle weakness and fatigability. Measurement of these antibodies can be of considerable value in disease diagnosis and management.
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Acetylcholine Receptor Antibody (AChR Ab) ELISA Kit
AChR Ab 适用: 人 Colorimetric Sandwich ELISA 78.125-5000 pM/L Plasma, Serum
Acetylcholine Receptor Antibody (AChR Ab) ELISA KitAChR Ab 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pmol/L - 4000 pmol/L Plasma, Serum, Tissue Homogenate
Acetylcholine Receptor Antibody (AChR Ab) ELISA KitAChR Ab 适用: 人 Colorimetric Indirect ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Acetylcholine Receptor Antibody (AChR Ab) ELISA KitAChR Ab 适用: 犬 Colorimetric Sandwich ELISA 3.75-240 ng/mL Plasma, Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 样本量
- 100 μL
- 实验流程
- The assay system depends on the ability of AChR Abs in human serum to bind to similar sites on the AChR as various monoclonal antibodies such as MAb1 (coated on ELISA plate wells) and/or MAb2 and/or MAb3 (which are labeled with biotin). In the absence of AChR Abs a complex is formed between MAb1 coated on the plate wells, the AChR and MAb2 and MAb3 biotin. MAb2 and MAb3 biotin bound are then detected by addition of streptavidin peroxidase (SA-POD), substrate (TMB) and stop solution. In the presence of AChR Abs the formation of the MAb1-AChR-MAb2/MAb3 biotin complex is inhibited, resulting in less SA-POD being bound and a reduction in final absorbance at 450nm. The higher the concentration of AChR Abs in the test serum the greater the inhibition of MAb biotin binding.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原 See all AChR Ab products
- AChR Ab (Acetylcholine Receptor Antibody (AChR Ab))
- 别名
- Anti-AChR (Acetylcholine Receptor) (AChR Ab 产品)
- 物质类
- Antibody, Antibody
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