RIPK1 ELISA 试剂盒
Quick Overview for RIPK1 ELISA 试剂盒 (ABIN2044401)
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检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.31 ng/mL
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灵敏度
- < 0.112 ng/mL
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Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) ELISA Kit
RIPK1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) ELISA KitRIPK1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) ELISA KitRIPK1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) ELISA KitRIPK1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) ELISA KitHigh Sensitivity RIPK1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Receptor Interacting Serine Threonine Kinase 1 (RIPK1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Interacting Serine Threonine Kinase 1 (RIPK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Interacting Serine Threonine Kinase 1 (RIPK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Interacting Serine Threonine Kinase 1 (RIPK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Receptor Interacting Serine Threonine Kinase 1 (RIPK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))
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别名
- Receptor Interacting Serine Threonine Kinase 1 (RIPK1)
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途径
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Activation of Innate immune Response, Inositol Metabolic Process, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome, Ubiquitin Proteasome Pathway
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