IGF1R ELISA 试剂盒
Quick Overview for IGF1R ELISA 试剂盒 (ABIN2043753)
抗原
See all IGF1R ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.057 ng/mL
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板类型
- Pre-coated
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实验流程
- Insulin Like Growth Factor 1 Receptor (IGF1R) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 1 Receptor (IGF1R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 1 Receptor (IGF1R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 1 Receptor (IGF1R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Insulin Like Growth Factor 1 Receptor (IGF1R) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))
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别名
- Insulin Like Growth Factor 1 Receptor (IGF1R)
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途径
- RTK signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy
抗原 See all IGF1R ELISA试剂盒
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