IGF1R ELISA 试剂盒
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Quick Overview for IGF1R ELISA 试剂盒 (ABIN6720107)
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See all IGF1R ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0457
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Supplier
- Rockland
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原理
- Human IGF1R Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: E31-N932 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Insulin-Like Growth Factor 1 Receptor (IGF1R) ELISA Kit
IGF1R 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Insulin-Like Growth Factor 1 Receptor (IGF1R) ELISA KitIGF1R 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 1 Receptor (IGF1R) ELISA KitIGF1R 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 1 Receptor (IGF1R) ELISA KitIGF1R 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Insulin-Like Growth Factor 1 Receptor (IGF1R) ELISA KitIGF1R 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Insulin-Like Growth Factor 1 Receptor (IGF1R) ELISA KitIGF1R 适用: 人 AA 31-932 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Insulin-Like Growth Factor 1 Receptor (IGF1R) ELISA KitIGF1R 适用: 人 Colorimetric Sandwich ELISA 6 pg/mL - 6000 pg/mL Cell Culture Supernatant, Plasma, Serum
Insulin-Like Growth Factor 1 Receptor (IGF1R) ELISA KitIGF1R 适用: 人 Colorimetric Sandwich ELISA 250-16000 pg/mL Cell Lysate, Plasma, Serum
Insulin-Like Growth Factor 1 Receptor (IGF1R) ELISA KitIGF1R 适用: 人 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor 1 Receptor (IGF1R) ELISA KitIGF1R 适用: 人 Colorimetric Sandwich ELISA 218.75 pg/mL - 14000 pg/mL Cell Culture Supernatant, Plasma, Serum
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说明
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Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human IGF1R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IGF1R standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 5.4%, 4.8%, 7.5%
Inter-Assay Precision (CV%): 7%, 6.3%, 8.9% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))
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别名
- IGF1R
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背景
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Background: IGF1R, also called IGFR or insulin-like growth factor 1 receptor, is a protein that found on the surface of human cells. This gene is mapped to 15q26.3. IGF1R belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in growth and continues to have anabolic effects in adults - meaning that it can induce hypertrophy of skeletal muscle and other target tissues. IGF1R also plays a critical role in transformation events. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.
Gene Full Name: insulin like growth factor 1 receptor
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基因ID
- 3480
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NCBI登录号
- NP_000866
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UniProt
- P08069
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途径
- RTK signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy
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