CYP27A1 ELISA 试剂盒
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- 抗原 See all CYP27A1 ELISA试剂盒
- CYP27A1 (Cytochrome P450, Family 27, Subfamily A, Polypeptide 1 (CYP27A1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.15-10 ng/mL
- 最低检测浓度
- 0.15 ng/mL
- 应用范围
- ELISA
- 品牌
- BioAssay™
- 灵敏度
- < 0.057 ng/mL
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- 板类型
- Pre-coated
- 实验流程
- Cytochrome P450 27A1 (CYP27A1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 27A1 (CYP27A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 27A1 (CYP27A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 27A1 (CYP27A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cytochrome P450 27A1 (CYP27A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
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- 抗原 See all CYP27A1 ELISA试剂盒
- CYP27A1 (Cytochrome P450, Family 27, Subfamily A, Polypeptide 1 (CYP27A1))
- 别名
- Cytochrome P450 27A1 (CYP27A1) (CYP27A1 产品)
- 别名
- CP27 ELISA Kit, CTX ELISA Kit, CYP27 ELISA Kit, Cyp27 ELISA Kit, P450C27 ELISA Kit, 1300013A03Rik ELISA Kit, cytochrome P450 family 27 subfamily A member 1 ELISA Kit, cytochrome P450, family 27, subfamily a, polypeptide 1 ELISA Kit, cytochrome P450, family 27, subfamily A, polypeptide 1 ELISA Kit, sterol 26-hydroxylase, mitochondrial ELISA Kit, CYP27A1 ELISA Kit, Cyp27a1 ELISA Kit, cyp27a1 ELISA Kit
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