CARTPT ELISA 试剂盒
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- 抗原 See all CARTPT ELISA试剂盒
- CARTPT (CART Prepropeptide (CARTPT))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.15-10 ng/mL
- 最低检测浓度
- 0.15 ng/mL
- 应用范围
- ELISA
- 品牌
- BioAssay™
- 灵敏度
- < 0.055 ng/mL
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- 板类型
- Pre-coated
- 实验流程
- Cocaine And Amphetamine Regulated Transcript (CART) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cocaine And Amphetamine Regulated Transcript (CART). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cocaine And Amphetamine Regulated Transcript (CART). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cocaine And Amphetamine Regulated Transcript (CART), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cocaine And Amphetamine Regulated Transcript (CART) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
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- 抗原 See all CARTPT ELISA试剂盒
- CARTPT (CART Prepropeptide (CARTPT))
- 别名
- Cocaine And Amphetamine Regulated Transcript (CART) (CARTPT 产品)
- 别名
- CART ELISA Kit, Cart ELISA Kit, CARTPT ELISA Kit, CART prepropeptide ELISA Kit, CARTPT ELISA Kit, Cartpt ELISA Kit
- 途径
- Hormone Transport, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Feeding Behaviour
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