Choline Acetyltransferase ELISA 试剂盒
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- 抗原 See all Choline Acetyltransferase (CHAT) ELISA试剂盒
- Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.15-10 ng/mL
- 最低检测浓度
- 0.15 ng/mL
- 应用范围
- ELISA
- 品牌
- BioAssay™
- 灵敏度
- < 0.056 ng/mL
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- 板类型
- Pre-coated
- 实验流程
- Choline Acetyltransferase (ChAT) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Choline Acetyltransferase (ChAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Choline Acetyltransferase (ChAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Choline Acetyltransferase (ChAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Choline Acetyltransferase (ChAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C/-20 °C
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- 抗原 See all Choline Acetyltransferase (CHAT) ELISA试剂盒
- Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))
- 别名
- Choline Acetyltransferase (ChAT) (CHAT 产品)
- 别名
- CHOACTASE ELISA Kit, CMS1A ELISA Kit, CMS1A2 ELISA Kit, ChoAcTase ELISA Kit, B230380D24Rik ELISA Kit, choline O-acetyltransferase ELISA Kit, choline O-acetyltransferase a ELISA Kit, choline acetyltransferase ELISA Kit, carnitine O-acetyltransferase ELISA Kit, CHAT ELISA Kit, chata ELISA Kit, Chat ELISA Kit, PGTG_00551 ELISA Kit, LOC100533322 ELISA Kit
- 途径
- Skeletal Muscle Fiber Development
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