CHEK1 ELISA 试剂盒
This 小鼠 CHEK1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 CHEK1.
Quick Overview for CHEK1 ELISA 试剂盒 (ABIN2043125)
抗原
See all CHEK1 ELISA试剂盒
CHEK1
(Checkpoint Kinase 1 (CHEK1))
适用
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.15-10 ng/mL
应用范围
ELISA
-
-
最低检测浓度
- 0.15 ng/mL
-
灵敏度
- < 0.058 ng/mL
-
-
Checkpoint Kinase 1 (CHEK1) ELISA Kit
CHEK1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
-
-
板类型
- Pre-coated
-
实验流程
- Checkpoint Homolog (CHEK1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Checkpoint Homolog (CHEK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Checkpoint Homolog (CHEK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Checkpoint Homolog (CHEK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Checkpoint Homolog (CHEK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C/-20 °C
-
-
- CHEK1 (Checkpoint Kinase 1 (CHEK1))
-
别名
- Checkpoint Homolog (CHEK1)
-
途径
- p53 Pathway, Apoptosis, Cell Division Cycle, DNA Damage Repair
抗原 See all CHEK1 ELISA试剂盒
-
You are here:
