(1 reference)-
- 抗原
- Matrix Metalloproteinase
- 检测方法
- Fluorometric
- 应用范围
- Fluorescence Resonance Energy Transfer Microscopy (FRET), Activation (Act)
- 品牌
- SensoLyte®
- 产品特性
- The SensoLyte® 390 Generic MMP Assay Kit is optimized to detect the activity of a variety of MMPs, including MMP-1, 2, 7, 8, 9, 13, 14, 15, 16, and 24. This kit can be used for detecting generic MMP activity in biological samples or for high throughput screening of MMP inducers and inhibitors using purified MMPs. This kit provides an Mca/Dnp fluorescence resonance energy transfer (FRET) peptide as a MMP substrate. In the intact FRET peptide, the fluorescence of Mca is quenched by Dnp. Upon cleavage into two separate fragments by MMPs, the fluorescence of Mca is recovered, and can be monitored at excitation/emission wavelengths = 330 nm/390 nm. The assays are performed in a convenient 96-well microplate format.
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SensoLyte® 520 Generic MMP Activity Kit
FRET, Act Fluorometric
SensoLyte® Generic MMP Assay KitEAA Colorimetric
SensoLyte® 570 Generic MMP Assay KitFRET Fluorometric
SensoLyte® 520 MMP Substrate Sampler KitFRET Fluorometric
SensoLyte® 520 MMP Profiling KitFRET Fluorometric
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- 说明
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FRET-based Assay Kit
- 限制
- 仅限研究用
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- 注意事项
- Protect Components A and B from light.
- 储存条件
- -20 °C
- 储存方法
- Store all components at -20 °C. Components D and E can be stored at 4 °C for convenience.
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: "Sphingosine-1-phosphate protects endothelial glycocalyx by inhibiting syndecan-1 shedding." in: American journal of physiology. Heart and circulatory physiology, Vol. 306, Issue 3, pp. H363-72, (2014) (PubMed).
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: "Sphingosine-1-phosphate protects endothelial glycocalyx by inhibiting syndecan-1 shedding." in: American journal of physiology. Heart and circulatory physiology, Vol. 306, Issue 3, pp. H363-72, (2014) (PubMed).
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- 抗原
- Matrix Metalloproteinase
- 别名
- MMP
- 背景
- Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMPs play important roles in tumor development and invasion, as well in many diseases.
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