(4 references)-
- 抗原
- LDH
- 检测方法
- Fluorometric
- 应用范围
- Enzyme Activity Assay (EAA)
- 产品特性
- The SensoLyte® Cell Cytotoxicity Assay Kit uses resazurin as a sensitive fluorogenic indicator (Ex/Em=560 nm/590 nm upon conversion) to measure LDH activity. The assay can be performed in a mixed population of damaged and viable cells, but it only measures the LDH released from damaged cells. The cytoplasmic LDH in living cells produces little signals under assay condition. There is no need for extra steps to separate living cells and supernatant. The fluorescent signal is proportional to the number of damaged cells (up to 2.5X104 cell, r2>0.95) with the detection limit reaching 100 dead cells. The kit is suitable for high throughput screening of cytotoxicity of a variety of compounds. 384-well or 1536-well format can be used with minor modifications.
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- 说明
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Enzymatic Activity Assay kit
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Store all kit components at -20 °C. Components C and D can be stored at room temperature for convenience.
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: "In vitro cytotoxicity screening of water-dispersible metal oxide nanoparticles in human cell lines." in: Bioprocess and biosystems engineering, Vol. 33, Issue 1, pp. 21-30, (2009) (PubMed).
: "Diminished survival of human cytotrophoblast cells exposed to hypoxia/reoxygenation injury and associated reduction of heparin-binding epidermal growth factor-like growth factor." in: American journal of obstetrics and gynecology, Vol. 198, Issue 4, pp. 471.e1-7; discussion 471.e7-8, (2008) (PubMed).
: "Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells." in: Biology of reproduction, Vol. 77, Issue 1, pp. 53-60, (2007) (PubMed).
: "Human trophoblast survival at low oxygen concentrations requires metalloproteinase-mediated shedding of heparin-binding EGF-like growth factor." in: Development (Cambridge, England), Vol. 133, Issue 4, pp. 751-9, (2006) (PubMed).
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: "In vitro cytotoxicity screening of water-dispersible metal oxide nanoparticles in human cell lines." in: Bioprocess and biosystems engineering, Vol. 33, Issue 1, pp. 21-30, (2009) (PubMed).
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- 抗原
- LDH
- 背景
- The damage of cell membrane leads to the release of cytoplasmic enzymes. The measurement of released cytoplasmic lactate dehydrogenase (LDH) is a well-accepted assay to estimate cell membrane integrity and quantify cytotoxicity.
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