TACI ELISA 试剂盒
(1 reference)-
- 抗原 See all TACI (TNFRSF13B) ELISA试剂盒
- TACI (TNFRSF13B) (Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B))
- 抗原表位
- AA 2-166
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 93.7-6000 pg/mL
- 最低检测浓度
- 93.7 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFRSF13B/TACI
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: S2-T166 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: S2-T166 - Featured
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Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B) ELISA Kit
TNFRSF13B 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B) ELISA KitTNFRSF13B 适用: 小鼠 AA 2-128 Colorimetric Sandwich ELISA 46.9-3000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B) ELISA KitTNFRSF13B 适用: 人 Colorimetric Sandwich ELISA 93.7 pg/mL - 6000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
-
Sequence similarities: Contains 2 TNFR-Cys repeats.
Tissue Specificity: Highly expressed in spleen, thymus, small intestine and peripheral blood leukocytes. Expressed in resting B- cells and activated T-cells, but not in resting T-cells.
- 板类型
- Pre-coated
- 实验流程
- human TNFRSF13B ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF13B has been precoated onto 96-well plates. Standards(NSO, S2-T166) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF13B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFRSF13B amount of sample captured in plate.
- 实验流程
-
Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/m human TNFRSF13B standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TNFRSF13B standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(pg/ml): 527, Standard deviation: 19, CV(%): 3.6
- Sample 2: n=16, Mean(pg/ml): 2085, Standard deviation: 91.74, CV(%): 4.4
- Sample 3: n=16, Mean(pg/ml): 3561, Standard deviation: 185.2, CV(%): 5.2,
- Sample 1: n=24, Mean(pg/ml): 686, Standard deviation: 31.6, CV(%): 4.6
- Sample 2: n=24, Mean(pg/ml): 2664, Standard deviation: 151.8, CV(%): 5.7
- Sample 3: n=24, Mean(pg/ml): 3724, Standard deviation: 242.1, CV(%): 6.5
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
-
: "Characteristics of CD4+CD25+Foxp3+ regulatory T cells in patients with multiple organ dysfunction syndrome." in: Experimental and therapeutic medicine, Vol. 11, Issue 5, pp. 1908-1912, (2016) (PubMed).
-
: "Characteristics of CD4+CD25+Foxp3+ regulatory T cells in patients with multiple organ dysfunction syndrome." in: Experimental and therapeutic medicine, Vol. 11, Issue 5, pp. 1908-1912, (2016) (PubMed).
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- 抗原 See all TACI (TNFRSF13B) ELISA试剂盒
- TACI (TNFRSF13B) (Tumor Necrosis Factor Receptor Superfamily, Member 13B (TNFRSF13B))
- 别名
- TNFRSF13B (TNFRSF13B 产品)
- 别名
- CD267 ELISA Kit, CVID ELISA Kit, CVID2 ELISA Kit, RYZN ELISA Kit, TACI ELISA Kit, TNFRSF14B ELISA Kit, TNFRSF13B ELISA Kit, 1200009E08Rik ELISA Kit, Taci ELISA Kit, TNF receptor superfamily member 13B ELISA Kit, tumor necrosis factor receptor superfamily, member 13b ELISA Kit, TNFRSF13B ELISA Kit, Tnfrsf13b ELISA Kit
- 背景
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Protein Function: Receptor for TNFSF13/APRIL and TNFSF13B/TALL1/BAFF/BLYS that binds both ligands with similar high affinity. Mediates calcineurin-dependent activation of NF-AT, as well as activation of NF-kappa-B and AP-1. Involved in the stimulation of B- and T- cell function and the regulation of humoral immunity. .
Background: Tumor necrosis factor receptor superfamily member 13B, also known as TNFRSF13B or more commonly as TACI, is a transmembrane receptor protein found predominantly on the surface of B cells, which are an important part of the immune system. TACI controls T cell-independent B cell antibody responses, isotype switching, and B cell homeostasis. TACI is a lymphocyte-specific member of the tumor necrosis factor(TNF) receptor superfamily. It was originally discovered because of its ability to interact with calcium-modulator and cyclophilin ligand(CAML). TACI was later found to play a crucial role in humoral immunity by interacting with two members of the TNF family: BAFF and APRIL. These proteins signal through TACI inducing activation of several transcription factors including NFAT, AP-1, and NF-kappa-B which then modulate cellular activities. Defects in the function of TACI can lead to immune system diseases.
Synonyms: Tumor necrosis factor receptor superfamily member 13B,Transmembrane activator and CAML interactor,CD267,TNFRSF13B,TACI,
Full Gene Name: Tumor necrosis factor receptor superfamily member 13B
Cellular Localisation: Membrane, Single-pass type III membrane protein. - 基因ID
- 23495
- UniProt
- O14836
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