FSTL3 ELISA 试剂盒
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- 抗原 See all FSTL3 ELISA试剂盒
- FSTL3 (Follistatin-Like 3 (Secreted Glycoprotein) (FSTL3))
- 抗原表位
- AA 27-263
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human FLRG/FSTL3
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine, Milk
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: M27-V263 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: M27-V263 - Featured
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- Discover our top product FSTL3 ELISA Kit
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Follistatin-Like 3 (Secreted Glycoprotein) (FSTL3) ELISA Kit
FSTL3 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Follistatin-Like 3 (Secreted Glycoprotein) (FSTL3) ELISA KitFSTL3 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Follistatin-Like 3 (Secreted Glycoprotein) (FSTL3) ELISA KitFSTL3 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Contains 2 follistatin-like domains.
Tissue Specificity: Expressed in a wide range of tissues. .
- 板类型
- Pre-coated
- 实验流程
- human FLRG ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FLRG has been precoated onto 96-well plates. Standards(NSO, M27-V263) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FLRG is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FLRG amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human FLRG standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), urine or human milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human FLRG standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 0.52, Standard deviation: 0.022, CV(%): 4.2
- Sample 2: n=16, Mean(ng/ml): 1.13, Standard deviation: 0.044, CV(%): 3.9
- Sample 3: n=16, Mean(ng/ml): 2.28, Standard deviation: 0.093, CV(%): 4.1,
- Sample 1: n=24, Mean(ng/ml): 0.57, Standard deviation: 0.043, CV(%): 7.5
- Sample 2: n=24, Mean(ng/ml): 1.24, Standard deviation: 0.084, CV(%): 6.8
- Sample 3: n=24, Mean(ng/ml): 2.36, Standard deviation: 0.175, CV(%): 7.4
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all FSTL3 ELISA试剂盒
- FSTL3 (Follistatin-Like 3 (Secreted Glycoprotein) (FSTL3))
- 别名
- FSTL3 (FSTL3 产品)
- 别名
- E030038F23Rik ELISA Kit, Flrg ELISA Kit, FLRG ELISA Kit, FSRP ELISA Kit, follistatin-like 3 ELISA Kit, follistatin like 3 ELISA Kit, Fstl3 ELISA Kit, FSTL3 ELISA Kit
- 背景
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Protein Function: Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling, more effective on activin A than on activin B. Involved in bone formation, inhibits osteoclast differentiationc. Involved in hematopoiesis, involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10. .
Background: FSTL3(Follistatin-Like 3) also known as FLRG or FOLLISTATIN-RELATED GENE, is a member of the follistatin-module protein family, which is composed of extracellular matrix-associated glycoproteins thought to act in a paracrine manner to bind morphogens or growth/differentiation factors and regulate their activity during development. The FSTL3 gene extends over 7 kbp and contains 5 exons. The FSTL3 gene was localized to chromosome 19p13. This gene involved in hematopoiesis and in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. It may have a role in leukemogenesis.
Synonyms: Follistatin-related protein 3,Follistatin-like protein 3,Follistatin-related gene protein,FSTL3,FLRG,UNQ674/PRO1308,
Full Gene Name: Follistatin-related protein 3
Cellular Localisation: Isoform 1: Secreted. - 基因ID
- 10272
- UniProt
- O95633
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