IL-6 Receptor ELISA 试剂盒
Quick Overview for IL-6 Receptor ELISA 试剂盒 (ABIN1672798)
抗原
See all IL-6 Receptor (IL6R) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-6R alpha
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: sf21
Immunogen sequence: L20-D358 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: sf21
Immunogen sequence: L20-D358
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Interleukin 6 Receptor (IL6R) ELISA Kit
IL6R 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Interleukin 6 Receptor (IL6R) ELISA KitIL6R 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Interleukin 6 Receptor (IL6R) ELISA KitIL6R 适用: 人 Colorimetric Sandwich ELISA 1.88 ng/mL - 120 ng/mL Plasma, Serum, Tissue Homogenate, Urine
Interleukin 6 Receptor (IL6R) ELISA KitIL6R 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 6 Receptor (IL6R) ELISA KitIL6R 适用: 小鼠 Colorimetric Sandwich ELISA 6-1500 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 6 Receptor (IL6R) ELISA KitIL6R 适用: 人 Colorimetric Sandwich ELISA 16-1000 pg/mL Cell Lysate, Plasma, Serum
Interleukin 6 Receptor (IL6R) ELISA KitIL6R 适用: 人, 猕猴 Colorimetric Sandwich ELISA 12-3000 pg/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- human IL-6R alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-6R alpha has been precoated onto 96-well plates. Standards(sf21, L20-D358) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-6R alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-6R alpha amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL-6R alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-6R alpha standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 115, Standard deviation: 9.2, CV(%): 8
- Sample 2: n=16, Mean(pg/ml): 768, Standard deviation: 26.11, CV(%): 3.4
- Sample 3: n=16, Mean(pg/ml): 1558, Standard deviation: 60.76, CV(%): 3.9,
- Sample 1: n=24, Mean(pg/ml): 124, Standard deviation: 10.54, CV(%): 8.5
- Sample 2: n=24, Mean(pg/ml): 845, Standard deviation: 47.32, CV(%): 5.6
- Sample 3: n=24, Mean(pg/ml): 1334, Standard deviation: 81.37, CV(%): 6.1
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))
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别名
- IL6R
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背景
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Background: IL6R alpha(IL6RA) also known as CD126 or IL6R, is a type I cytokine receptor. The IL6 receptor is a protein complex consisting of a IL-6 receptor subunit(IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit is also shared by many other cytokines. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor. Ligand binding did not appear to affect IL6R dimerization status. IL6R dimerization occurs both on the cell surface and in solution.
Synonyms: Interleukin 6 receptor ,Interleukin 6 receptor, isoform CRA_a ,IL6R ,hCG_1810911 ,
Full Gene Name: interleukin 6 receptor
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基因ID
- 3570
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UniProt
- A0N0L5
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途径
- JAK/STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints
抗原 See all IL-6 Receptor (IL6R) ELISA试剂盒
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