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IL-6 Receptor ELISA 试剂盒

IL6R 适用: 人, 猕猴 Colorimetric Sandwich ELISA 12-3000 pg/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN2748263
发货至: 中国
  • 抗原 See all IL-6 Receptor (IL6R) ELISA试剂盒
    IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))
    适用
    • 7
    • 4
    • 3
    • 2
    • 2
    • 1
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    • 1
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    人, 猕猴
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    12-3000 pg/mL
    最低检测浓度
    12 pg/mL
    应用范围
    ELISA
    原理
    Rhesus Monkey IL-6 R ELISA Kit for cell culture supernatants, plasma, and serum samples.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair detects Rhesus Monkey and human IL-6R. Other species not determined.
    灵敏度
    12 pg/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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    Discover our top product IL6R ELISA Kit
  • 应用备注
    Recommended Dilution for serum and plasma samples30 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    实验流程

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all IL-6 Receptor (IL6R) ELISA试剂盒
    IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))
    别名
    IL-6R (IL6R 产品)
    别名
    IL6R ELISA Kit, il-6ra ELISA Kit, CD126 ELISA Kit, IL-6R-1 ELISA Kit, IL-6RA ELISA Kit, IL6Q ELISA Kit, IL6RA ELISA Kit, IL6RQ ELISA Kit, gp80 ELISA Kit, IL6R1 ELISA Kit, Il6ra ELISA Kit, IL-6Ralpha ELISA Kit, IL-6R ELISA Kit, Il6r ELISA Kit, interleukin 6 receptor ELISA Kit, interleukin 6 receptor, alpha ELISA Kit, IL6R ELISA Kit, il6r ELISA Kit, Il6r ELISA Kit, Il6ra ELISA Kit
    背景
    Interleukin-6 receptor subunit alpha isoform 1 (Fragment)
    UniProt
    H9FAX3
    途径
    JAK/STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints
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