小鼠 IgG2b,kappa isotype control (DyLight 650)
FACS, WB, ICC, IHC (fro), IHC (p)
Monoclonal
MPC-11
IgG2b kappa
DyLight 650
1 image
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- 抗原
- IgG2b,kappa
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宿主
- 小鼠
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克隆类型
- 单克隆
- 标记
- DyLight 650
- 应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
- 无交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Purified antibody is conjugated with DyLight® 650 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- 免疫原
- KLH-coupled trinitrophenol
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克隆位点
- MPC-11
- 亚型
- IgG2b kappa
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Mouse IgG2b,kappa isotype control (DyLight 488)
FACS, WB, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa DyLight 488
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- 应用备注
- Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.
- 限制
- 仅限研究用
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- 浓度
- 0.1 mg/mL
- 缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- 抗原
- IgG2b,kappa
- 别名
- IgG2b kappa
- 物质类
- Antibody
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