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Llama IgG isotype control (FITC)

FACS, WB, FLISA, FM IgG FITC
产品编号 ABIN5633240
发货至: 中国
  • 抗原 See all IgG products
    IgG
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    Llama
    标记
    FITC
    应用范围
    Flow Cytometry (FACS), Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
    纯化方法
    Llama IgG has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG1 shows a band of ~150 kDa whereas the reduced form exhibits ~55 kDa (heavy chain) and ~25 kDa (light chain), non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa, non-reduced llama IgG3 shows a band of ~100 kDa whereas the reduced form exhibits ~43 kDa.
    亚型
    IgG
    Labeling Ratio
    3.2
  • 应用备注

    Flow Cytometry Dilution: User Optimized

    Application Note: Llama IgG Fluorescein can be utilized as a control or standard reagent in SDS, Western Blotting, Flow Cytometry, Immunofluorescence, and FLISA experiments. Specific conditions for reactivity should be optimized by the end user.

    Western Blot Dilution: User Optimized

    FLISA Dilution: User Optimized

    IF Microscopy Dilution: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
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