The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The FITC Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3e, clone 145-2C11, anti-mouse Ly-6G/Ly-6C, clone RB6-8C5, anti-mouse CD11b, clone M1/70, anti-mouse CD45R/B220, clone RA3-6B2, anti-mouse TER-119/Erythroid cells, clone Ter-119. FITC Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin. Materials provided: 1. FITC anti-mCD3/ FITC anti-mGr-1/ FITC anti-mCD11b/ FITC anti-mCD45R(B220)/ FITC anti-mTer-119, 25 or 100 tests 2. FITC Armenian hamster IgG/ FITC Rat IgG2b/ FITC Rat IgG2a isotype controls, 25 or 100 tests Materials not provided: Cell Staining Buffer (Cat. No. 420201)
纯化方法
The antibodies are purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution are free of unconjugated FITC.
克隆位点
145-2c11-RB6-8C5-RA3-6B2-Ter-119-M1-70
亚型
IgG2b, IgG2a, IgG
应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
This two-reagent set contains FITC conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.
注意事项
Protect from prolonged exposure to light. Do not freeze.
储存条件
4 °C
储存方法
The antibody solution should be stored undiluted between 2°C and 8°C.