The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The PE Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3ε, clone 145-2C11, anti-mouse Ly-6G/Ly-6C, clone RB6-8C5, anti-mouse CD11b, clone M1/70, anti-mouse CD45R/B220, clone RA3-6B2, anti-mouse TER-119/Erythroid cells, clone Ter-119. PE Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin. Materials provided: 1. PE anti-mCD3ε/ PE anti-mGr-1/ PE anti-mCD11b/ PE anti-mCD45R(B220)/ PE anti-mTer-119, 25 tests 2. PE Armenian hamster IgG/ PE Rat IgG2b/ PE Rat IgG2a isotype controls, 25 tests Materials not provided: Cell Staining Buffer (Cat. No. 420201)
纯化方法
The antibodies were purified by affinity chromatography, and conjugated with PE under optimal conditions. The solutions are free of unconjugated PE and unconjugated antibody.
克隆位点
145-2c11-RB6-8C5-RA3-6B2-Ter-119-M1-70
亚型
IgG2b, IgG2a, IgG
应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
This two-reagent set contains PE conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.
注意事项
Do not freeze.
储存条件
4 °C
储存方法
It should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.