We are preparing the requested document. Please wait, this may take a while...!
|抗原||Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) 抗体|
|适用||人, 小鼠, 大鼠 其他选择|
|标记||This ERN1 antibody is un-conjugated 其他选择|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
|Supplier||Log in to see|
ERN1抗体详情ERN1目标详情 使用细节 贮存及处理 图像
|特异性||IRE1p antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human IRE1p.|
|纯化方法||Affinity Chromatography purified via peptide column|
|免疫原||16 amino acid synthetic peptide from near the carboxy terminus of Human IRE1p|
ERN1目标详情ERN1抗体详情 使用细节 贮存及处理 图像 回页首
|别名||ERN1 / IRE1 (ERN1 Antibody 摘要)|
|背景||Accumulation of malfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94 (1,2). These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF6 (3,4) but ER stress causes their dissociation. This allows IRE1p, a serine-threonine protein kinase to transduce the unfolded protein signal from the ER to the nucleus. IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation (5). Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER (6). Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.Synonyms: Endoplasmic reticulum-to-nucleus signaling 1, IRE1a, IRE1p, Inositol-requiring protein 1, Ire1-alpha, Serine/threonine-protein kinase/endoribonuclease IRE1|
|途径||ER-Nucleus Signaling, Unfolded protein response|
使用细节ERN1抗体详情 ERN1目标详情 贮存及处理 图像 回页首
ELISA. Western Blot: 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
贮存及处理ERN1抗体详情 ERN1目标详情 使用细节 图像 回页首
|缓冲液||PBS containing 0.02 % Sodium Azide|
|注意事项||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|注意事项||Avoid repeated freezing and thawing.|
|储存条件||4 °C/-20 °C|
|储存方法||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|