anti-小鼠 PMS2 抗体 for Immunofluorescence

Recommended PMS2 Antibody (supplied by: Log in to see )

抗原
PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2) 抗体
  • HNPCC4
  • PMS2CL
  • PMSL2
  • AW555130
  • Pmsl2
  • PMS1 homolog 2, mismatch repair system component
  • PMS1 homolog2, mismatch repair system component
  • mismatch repair endonuclease PMS2
  • PMS2
  • Pms2
  • LOC463257
  • LOC107984056
适用
人, 小鼠
125
41
19
2
2
2
2
1
1
1
1
1
1
1
1
宿主

71
54
1
克隆类型
多克隆
标记
This PMS2 antibody is un-conjugated
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
98
54
53
35
27
19
12
8
3
2
2
1
Supplier
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产品编号 ABIN6258634
$ 390.77
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2882900 IF WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5661542 IC IF WB Rabbit Log in to see Polyclonal 0

General

抗原 PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2) 抗体
适用 人, 小鼠
(125), (41), (19), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
宿主
(71), (54), (1)
克隆类型
多克隆
标记 This PMS2 antibody is un-conjugated
(3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(98), (54), (53), (35), (27), (19), (12), (8), (3), (2), (2), (1)
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PMS2抗体详情

PMS2目标详情 使用细节 贮存及处理 图像
特异性 PMS2 antibody detects endogenous levels of total PMS2
交叉反应 人, 小鼠
纯化方法 The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
免疫原 A synthesized peptide
亚型 IgG
Plasmids, Primers & others

PMS2目标详情

PMS2抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 PMS2 (PMS2 Antibody 摘要)
背景

Description: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.

Gene: PMS2

分子量 110 kDa
基因ID 5395
UniProt P54278
途径 DNA Damage Repair, Production of Molecular Mediator of Immune Response

使用细节

PMS2抗体详情 PMS2目标详情 贮存及处理 图像 回页首
应用备注 WB 1:500-1:2000, IF/ICC 1:100-1:500
限制 仅限研究用

贮存及处理

PMS2抗体详情 PMS2目标详情 使用细节 图像 回页首
状态 Liquid
浓度 1 mg/mL
缓冲液 Rabbit IgG in phosphate buffered saline,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

图像

PMS2抗体详情 PMS2目标详情 使用细节 贮存及处理 回页首
厂商提供的图像
Western Blotting (WB) image for anti-PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2) antibody (ABIN6258634) Western blot analysis of PMS2 expression in Hela cell lysate,The lane on the left is ...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2) antibody (ABIN6258634) ABIN6273522 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeab...