SDS-PAGE Running Gel Buffer (10X)
SDS
10 X
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- 应用范围
- SDS-PAGE (SDS)
- 产品特性
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SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearize proteins and to impart a negative charge to linearized proteins.
Synonyms: Sodium dodecyl sulfate polyacrylamide running buffer, Protein Gel Running Buffer, polyacrylamide gel running buffer.
Sterilization: Sterile Aseptic Filter
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- 应用备注
- SDS-PAGE running buffer is suitable for laboratory involved in protein biochemistry. This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water (or equivalent) to its final working concentration. No pH adjustment is required. 10X SDS-PAGE Running Buffer consists of 0.25 M Tris HCl, 1.92 M Glycine and 1% (w/v) Sodium Dodecyl Sulfate (SDS) pH 8.3. Meticulously prepared using ultra-pure reagents dissolved in highly polished pharmaceutical grade deionized water.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 10 X
- 储存条件
- RT
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