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E2F1 抗体 (AA 69-223)

Name: E2F1 抗体 (AA 69-223)
Brand: antibodies-online
SKU: ABIN969516
Availability: OutOfStock

This anti-E2F1 antibody is a 小鼠单克隆 antibody detecting E2F1 in WB, ELISA, IHC 和 FACS. Suitable for 人和小鼠. This Primary Antibody has been cited in 4+ publications.

Western blot analysis using E2F1 mouse mAb against HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate. (E2F1 抗体 (AA 69-223))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (E2F1 抗体 (AA 69-223))

ELISA

Flow cytometric analysis of HeLa cells using E2F1 mouse mAb (green) and negative control (red). (E2F1 抗体 (AA 69-223))

FACS

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using E2F1 mouse mAb with DAB staining. (E2F1 抗体 (AA 69-223))

IHC

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using E2F1 mouse mAb with DAB staining. (E2F1 抗体 (AA 69-223))

IHC

Western blot analysis using E2F1 mAb against human E2F1 recombinant protein. (E2F1 抗体 (AA 69-223))

6 images

(4 references)

产品编号 ABIN969516

发货至: 中国

Quick Overview for E2F1 抗体 (AA 69-223) (ABIN969516)

抗原

See all E2F1 抗体

E2F1 (E2F Transcription Factor 1 (E2F1))

适用

人, 小鼠

宿主

小鼠

克隆类型

单克隆

标记

This E2F1 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

克隆位点

8G9

抗原表位
25

24

20

15

15

10

8

8

8

6

5

5

4

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 69-223

原理

E2F1 Antibody

纯化方法

Purified antibody

免疫原

Purified recombinant fragment of human E2F1 (AA: 69-223) expressed in E. Coli.

亚型

IgG1
anti-E2F Transcription Factor 1 (E2F1) antibody
E2F1 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 兔 Monoclonal 1D12 unconjugated Recombinant Antibody

anti-E2F Transcription Factor 1 (E2F1) (AA 348-437) antibody
E2F1 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2E10 unconjugated

anti-E2F Transcription Factor 1 (E2F1) (AA 101-180) antibody
E2F1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (p), IF (cc), FACS, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-E2F Transcription Factor 1 (E2F1) (AA 133-362) antibody
E2F1 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-E2F Transcription Factor 1 (E2F1) antibody
E2F1 适用: 人 WB, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-E2F Transcription Factor 1 (E2F1) (AA 342-371) antibody
E2F1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-E2F Transcription Factor 1 (E2F1) (pSer364) antibody
E2F1 适用: 人 WB, ELISA, IF (p), IF (cc), FACS, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-E2F Transcription Factor 1 (E2F1) (pThr433) antibody
E2F1 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-E2F Transcription Factor 1 (E2F1) (C-Term) antibody
E2F1 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-E2F Transcription Factor 1 (E2F1) antibody
E2F1 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated

应用备注

ELISA: 1/10000
FCM: 1/200 - 1/400

限制

仅限研究用
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Zhou, Jiang, Xia, Zhang: "Comparison of the therapeutic effects of lobaplatin and carboplatin on retinoblastoma in vitro and in vivo." in: International journal of oncology, Vol. 57, Issue 3, pp. 697-706, (2020) (PubMed).

Okazaki, Matsunaga, Okazaki, Utoguchi, Suzuki, Maruyama, Koyanagi, Ohdo: "Circadian rhythm of transferrin receptor 1 gene expression controlled by c-Myc in colon cancer-bearing mice." in: Cancer research, Vol. 70, Issue 15, pp. 6238-46, (2010) (PubMed).

Gurtner, Fuschi, Martelli, Manni, Artuso, Simonte, Ambrosino, Antonini, Folgiero, Falcioni, Sacchi, Piaggio: "Transcription factor NF-Y induces apoptosis in cells expressing wild-type p53 through E2F1 upregulation and p53 activation." in: Cancer research, Vol. 70, Issue 23, pp. 9711-20, (2010) (PubMed).

Locatelli, Bosotti, Ciomei, Brasca, Calogero, Mercurio, Fiorentini, Bertolotti, Scacheri, Scaburri, Galvani, Pesenti, De Baere, Soria, Lazar, Isacchi: "Transcriptional analysis of an E2F gene signature as a biomarker of activity of the cyclin-dependent kinase inhibitor PHA-793887 in tumor and skin biopsies from a phase I clinical study." in: Molecular cancer therapeutics, Vol. 9, Issue 5, pp. 1265-73, (2010) (PubMed).
抗原

E2F1 (E2F Transcription Factor 1 (E2F1))

别名

E2F1

背景

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

分子量

46.9 kDa

基因ID

1869

UniProt

Q01094

途径

p53 Pathway, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy