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CTCF 抗体 (AA 184-290)

Name: CTCF 抗体 (AA 184-290)
SKU: ABIN968757
Availability: OutOfStock

This anti-CTCF antibody is a 小鼠单克隆 antibody detecting CTCF in WB 和 IF. Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 4+ publications.

Western blot analysis of CTCF on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). (CTCF 抗体 (AA 184-290))

Immunofluorescence staining of A431 cells (Human epithelial carcinoma, ATCC CRL-1555). (CTCF 抗体 (AA 184-290))

2 images

(4 references)

产品编号 ABIN968757

发货至: 中国

Datasheet as PDF

Quick Overview for CTCF 抗体 (AA 184-290) (ABIN968757)

抗原

See all CTCF 抗体

CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

适用

人, 小鼠, 大鼠

宿主

小鼠

克隆类型

单克隆

标记

This CTCF antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF)

克隆位点

48-CTCF

Discover our top product CTCF Primary Antibody

抗原表位
5

4

4

2

2

2

1

1

1

1

1

1

1

1

1
AA 184-290

交叉反应

小鼠, 大鼠

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human CTCF aa. 184-290

亚型

IgG1
anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 1-260) antibody
CTCF 适用: 人 WB, IHC, IF, IP, ChIP 宿主: 兔 Polyclonal unconjugated

anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) antibody
CTCF 适用: 人 WB, ChIP-seq, CUT&RUN 宿主: 兔 Monoclonal unconjugated Recombinant Antibody

anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) antibody
CTCF 适用: 人 WB, IF, IP, ChIP 宿主: 兔 Monoclonal unconjugated

anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (C-Term) antibody
CTCF 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 1-260) antibody
CTCF 适用: 人 WB, IHC, ELISA, ChIP 宿主: 兔 Polyclonal unconjugated

anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 334-364) antibody
CTCF 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB53773 unconjugated

anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (N-Term) antibody
CTCF 适用: 人, 小鼠, 大鼠, Cow, 犬, 兔, 马, 猴, 豚鼠, Bat, 小鸡, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 521-727) antibody
CTCF 适用: 人, 小鼠, 大鼠 WB, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated

anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 451-550) antibody
CTCF 适用: 人 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 1-100) antibody
CTCF 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

说明

Related Products: ABIN968537, ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20° C.
Bell, West, Felsenfeld: "The protein CTCF is required for the enhancer blocking activity of vertebrate insulators." in: Cell, Vol. 98, Issue 3, pp. 387-96, (1999) (PubMed).

Filippova, Lindblom, Meincke, Klenova, Neiman, Collins, Doggett, Lobanenkov: "A widely expressed transcription factor with multiple DNA sequence specificity, CTCF, is localized at chromosome segment 16q22.1 within one of the smallest regions of overlap for common deletions in breast and prostate cancers." in: Genes, chromosomes & cancer, Vol. 22, Issue 1, pp. 26-36, (1998) (PubMed).

Filippova, Fagerlie, Klenova, Myers, Dehner, Goodwin, Neiman, Collins, Lobanenkov: "An exceptionally conserved transcriptional repressor, CTCF, employs different combinations of zinc fingers to bind diverged promoter sequences of avian and mammalian c-myc oncogenes." in: Molecular and cellular biology, Vol. 16, Issue 6, pp. 2802-13, (1996) (PubMed).

Klenova, Nicolas, Paterson, Carne, Heath, Goodwin, Neiman, Lobanenkov: "CTCF, a conserved nuclear factor required for optimal transcriptional activity of the chicken c-myc gene, is an 11-Zn-finger protein differentially expressed in multiple forms." in: Molecular and cellular biology, Vol. 13, Issue 12, pp. 7612-24, (1994) (PubMed).
抗原

CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

别名

CTCF

背景

The family of nucleic acid-binding C2H2 type zinc finger transcription factors is divided into two classes. One class consists of small proteins (Gli1, Krox-20, WT1, Egr-1, and Sp1) with conserved zinc finger clusters of 3 to 5 units, while the other class (ZNF91, ZNF74, ZFP37, CTCF) can contain more than 10 zinc finger clusters. CTCF is a ubiquitously expressed, highly conserved transcription factor that contains 10 C2H2- and 1 C2H-type zinc-finger motifs. CTCF binds to and represses transcription at the promoter-proximal regions of c-myc oncogenes, while CTCF can bind to and activate transcription at the promoter for amyloid beta. Another mechanism of CTCF-mediated repression may include binding to insulator regions between enhancers and promoters resulting in enhancer blocking. In addition, CTCF gene may be a candidate tumor suppressor gene, since it localizes to a narrow cancer-associated chromosome region, and has been shown to have tumor-specific rearrangements in breast cancer patients. Thus, CTCF transcriptional regulation may be important for cell cycle progression, differentiation, apoptosis, and tumorigenesis. This antibody is routinely tested by western blot analysis.

分子量

140 kDa