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SLC9A1 抗体 (AA 682-801)

Name: SLC9A1 抗体 (AA 682-801)
SKU: ABIN968659
Availability: OutOfStock

SLC9A1 适用: 人, 大鼠, 小鼠, 犬 WB, IF 宿主: 小鼠 Monoclonal 54-NHE unconjugated

Western blot analysis for NHE on a HEK-293 cell lysate (Human embryonic kidney cells, ATCC CRL-1573). (SLC9A1 抗体 (AA 682-801))

Immunofluorescence staining of NIH/3T3 cells (Mouse embryo fibroblast cells, ATCC CRL-1658). (SLC9A1 抗体 (AA 682-801))

2 images

(5 references)

产品编号 ABIN968659

发货至: 中国

Datasheet as PDF

抗原 See all SLC9A1 抗体

SLC9A1 (Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1))
抗原表位
15

8

6

1

1

1

1

1

1

1

1

1
AA 682-801
适用
35

34

25

6

4

3

3

2

1

1

1

1

1

1

1

1

1
人, 大鼠, 小鼠, 犬
宿主
40

4

2
小鼠
Compatible Secondaries
克隆类型
42

4
单克隆
标记
20

4

3

2

2

2

2

2

2

2

1

1

1

1

1
This SLC9A1 antibody is un-conjugated
应用范围
36

18

13

13

8

5

4

4

3

2

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF)

交叉反应

人, 小鼠, 犬

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Rat NHE-1 aa. 682-801

克隆位点

54-NHE

亚型

IgG1

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Discover our top product SLC9A1 Primary Antibody
anti-Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1) (AA 543-815) antibody
SLC9A1 适用: 人, 大鼠, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1) (AA 36-102) antibody
SLC9A1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1) (AA 31-130) antibody
SLC9A1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2F5 unconjugated

anti-Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1) (1st Extracellular Loop), (AA 54-67) antibody
SLC9A1 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, FACS, LCI 宿主: 兔 Polyclonal unconjugated

anti-Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1) (AA 470-550) antibody
SLC9A1 适用: 大鼠, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1) (C-Term) antibody
SLC9A1 适用: 人, 大鼠, 小鼠, Pig, 兔, Cow, 马, 猴, Bat, Hamster WB 宿主: 兔 Polyclonal unconjugated

anti-Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1) (Middle Region) antibody
SLC9A1 适用: 人, 大鼠, 小鼠, Pig, 兔, Cow, 犬, 马, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1) antibody
SLC9A1 适用: Arabidopsis thaliana, Mangrove WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1) (C-Term), (N-Term) antibody
SLC9A1 适用: 人, 大鼠, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated

anti-Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1) (AA 470-550) antibody (Biotin)
SLC9A1 适用: 大鼠, 小鼠 WB, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal Biotin

说明

Related Products: ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20°C.
Zhao, Oxley, Smith, Follows, Green, Alexander: "DNA damage-induced Bcl-xL deamidation is mediated by NHE-1 antiport regulated intracellular pH." in: PLoS biology, Vol. 5, Issue 1, pp. e1, (2007) (PubMed).

Aharonovitz, Zaun, Balla, York, Orlowski, Grinstein: "Intracellular pH regulation by Na(+)/H(+) exchange requires phosphatidylinositol 4,5-bisphosphate." in: The Journal of cell biology, Vol. 150, Issue 1, pp. 213-24, (2000) (PubMed).

Cox, Lutz, Yang, Biemesderfer, Bronson, Fu, Aronson, Noebels, Frankel: "Sodium/hydrogen exchanger gene defect in slow-wave epilepsy mutant mice." in: Cell, Vol. 91, Issue 1, pp. 139-48, (1997) (PubMed).

Kandasamy, Yu, Harris, Boucher, Hanrahan, Orlowski: "Plasma membrane Na+/H+ exchanger isoforms (NHE-1, -2, and -3) are differentially responsive to second messenger agonists of the protein kinase A and C pathways." in: The Journal of biological chemistry, Vol. 270, Issue 49, pp. 29209-16, (1996) (PubMed).

Orlowski, Kandasamy, Shull et al.: "Molecular cloning of putative members of the Na/H exchanger gene family. cDNA cloning, deduced amino acid sequence, and mRNA tissue expression of the rat Na/H exchanger NHE-1 and two structurally ..." in: The Journal of biological chemistry, Vol. 267, Issue 13, pp. 9331-9, (1992) (PubMed).
抗原

SLC9A1 (Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1))

别名

NHE-1 (SLC9A1 产品)

别名

AT-NHX1 antibody, ATNHX antibody, ATNHX1 antibody, NA(+)/H(+) ANTIPORTER antibody, Na+/H+ exchanger 1 antibody, T21B4.60 antibody, T21B4_60 antibody, AW554487 antibody, Apnh antibody, Nhe1 antibody, swe antibody, NHE-1 antibody, NHE1 antibody, APNH antibody, Na+/H+ exchanger 1 antibody, solute carrier family 9 (sodium/hydrogen exchanger), member 1 antibody, solute carrier family 9 member A1 antibody, NHX1 antibody, Slc9a1 antibody, SLC9A1 antibody

背景

The extrusion of H+ in exchange for extracellular Na+ is important for many cellular processes, such as pH homeostasis, volume regulation, and transepithelial ion and water transport. Na+/H+ Exchangers (NHE) are integral membrane proteins that mediate electroneutral exchange of one Na+ ion for one H+ ion. Six NHE forms, NHE-1 thru -6, have been identified. NHE-1 and NHE-6 are widely expressed, while the other NHE forms have restricted expression. The common structure of all NHE forms includes 10-12 N-terminal membrane (M) spanning regions, a conserved M6 and M7 region that may participate in ion transport, and a large C-terminal cytoplasmic region that may be involved in the regulation of ion exchange activity. NHE-1, for example, contains 12 M regions plus domains for volume sensitivity, calmodulin-binding, CHP-binding, and PKC phosphorylation in the cytoplasmic region. Regulation of NHE-1 ion exchange activity may occur through phosphoinositide binding, as well as PKC- and PKA-dependent signaling pathways. Mutation of NHE-1 in mice causes neuronal death in the cerebellum and brainstem, leading to ataxia and seizures. Thus, NHE-1 is a ubiquitous NHE that is essential for normal brain function.
Although this antibody was developed against the NHE-1 antigen, investigators should note that crossreactivity to other NHE isoforms or variants may be possible.
Synonyms: Na+/H+ Exchangers

分子量

92 kDa

途径

Glycosaminoglycan Metabolic Process, Proton Transport