BAK1 抗体
(4 references)Quick Overview for BAK1 抗体 (ABIN967519)
抗原
See all BAK1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
品牌
- BD Pharmingen™
-
产品特性
-
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
-
免疫原
- Human Bak
-
亚型
- IgG1
-
-
anti-BCL2-Antagonist/killer 1 (BAK1) (AA 62-112) antibody
BAK1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-BCL2-Antagonist/killer 1 (BAK1) (AA 29-187) antibodyBAK1 适用: 人 ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 2H9H7 unconjugated
anti-BCL2-Antagonist/killer 1 (BAK1) (AA 22-211) antibodyBAK1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-BCL2-Antagonist/killer 1 (BAK1) antibodyBAK1 适用: 人 WB, ELISA, IHC, FACS, IP 宿主: 兔 Monoclonal 8D1 unconjugated Recombinant Antibody
anti-BCL2-Antagonist/killer 1 (BAK1) (AA 22-211) antibodyBAK1 适用: 人, 小鼠, 大鼠 WB, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-BCL2-Antagonist/killer 1 (BAK1) (AA 21-120) antibodyBAK1 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-BCL2-Antagonist/killer 1 (BAK1) (AA 1-14) antibodyBAK1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-BCL2-Antagonist/killer 1 (BAK1) (N-Term) antibodyBAK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-BCL2-Antagonist/killer 1 (BAK1) (N-Term) antibodyBAK1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-BCL2-Antagonist/killer 1 (BAK1) (AA 1-211) antibodyBAK1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Applications include western blot analysis (1-2 µg/ml). HeLa cells (ATCC CCL-2) are recommended as a positive control. HeLa cell lysate is also available as a ready-to-use positive western blot control (ABIN968535).
-
说明
-
Related Products: ABIN967389, ABIN968535
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.5 mg/mL
-
缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Store undiluted at 4°C.
-
-
-
: "Induction of apoptosis by the Bcl-2 homologue Bak." in: Nature, Vol. 374, Issue 6524, pp. 733-6, (1995) (PubMed).
: "Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak." in: Nature, Vol. 374, Issue 6524, pp. 736-9, (1995) (PubMed).
: "Bcl-2 and the regulation of programmed cell death." in: The Journal of cell biology, Vol. 124, Issue 1-2, pp. 1-6, (1994) (PubMed).
: "BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax." in: Nature, Vol. 369, Issue 6478, pp. 321-3, (1994) (PubMed).
-
: "Induction of apoptosis by the Bcl-2 homologue Bak." in: Nature, Vol. 374, Issue 6524, pp. 733-6, (1995) (PubMed).
-
- BAK1 (BCL2-Antagonist/killer 1 (BAK1))
-
别名
- Bak
-
背景
- Bak (for Bcl-2 homologous Antagonist/Killer) is a recently identified member of the Bcl-2 family. Bcl-2 family members are involved in mediating programmed cell death or apoptosis, and share two highly conserved functional regions, Bcl-2 homology 1 and 2 (BH1 and BH2). Several of the family members, including Bcl-2, act as inhibitors of apoptosis, whereas others such as Bax promote cell death. Like Bax promote cell death. Like Bax, Bak primarily promotes apoptosis. However, unlike Bax, Bak has also been shown to inhibit cell death. Bak inhibited both serum-starvation and drug induced apoptosis when overexpressed in an Epstein-Barr virus (EBV)-transformed cell line. However, Bax did not inhibit cell death under the same conditions. Bak mNRA has been identified in a wide variety of fetal and adult tissues, with the highest levels observed in heart and skeletal muscle. The apparently ubiquitous expression of Bak has lead to the suggestion that its function may be mediated by cell death inhibitory factors, particularly in cell types with a long life span. Bak migrates at a reduced molecular weight ~24 kDa. G317-2 reacts with human Bak. A synthetic peptide corresponding to the first 50 amino acids of human Bak was used as immunogen.
-
分子量
- 24 kDa
-
途径
- Apoptosis, Steroid Hormone Mediated Signaling Pathway, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
抗原
-
