1. Since applications vary, each investigator should titrate the reagent to obtain optimal results. 2. Please refer to us for technical protocols. 3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
纯化方法
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recognizes a triplet of membrane proteins of 74, 80 and 89 kDa. CD97 is strongly expressed on phytohemagglutinin (PHA)-activated T and B cells, virtually all monocytes and granulocytes, and several cell lines including HL-60, THP-1, and K562. It is weakly expressed onresting lymphocytes. VIM3b has been shown to be effective in western blot and immunoprecipitation. This antibody is routinely tested by flow cytometric analysis.