TNF alpha 抗体 (AA 115-130)
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- 抗原 See all TNF alpha 抗体
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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抗原表位
- AA 115-130
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This TNF alpha antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody reacts with TNFa.
- 纯化方法
- Concentrated.
- 免疫原
- Synthetic TNF-alpha 115-130 hexadecapeptide linked to carrier protein.
- 克隆位点
- B428
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product TNF alpha Primary Antibody
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- 应用备注
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Immunohistochemistry: 1:50-1:100
ELISA 1:500 -1:1000 (this antibody is not purified and can not be used to coat ELISA plates) .
Staining Procedure: This antibody can be used on frozen and formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:100 in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory. - 说明
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Cellular Localization: cytoplasmic and extracellular.
Recommended Positive Control: Prostate cancer - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.3 mg/ml.
- 缓冲液
- 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- 别名
- Tumor Necrosis Factor Alpha (TNF alpha 产品)
- 分子量
- 17 kDa
- 基因ID
- 7124
- 途径
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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