Western Bloting: 1/500 - 1/2000. ELISA: Propose dilution 1/10000. Not yet tested in other applications. Determining optimal working dilutions by titration test.
限制
仅限研究用
状态
Liquid
储存条件
4 °C
de Haan, Smeets, Vernooij, Vennema, Rottier: "Mapping of the coronavirus membrane protein domains involved in interaction with the spike protein." in: Journal of virology, Vol. 73, Issue 9, pp. 7441-52, (1999) (PubMed).
Blomhoff, Smedsrød, Eskild, Granum, Berg: "Preparation of isolated liver endothelial cells and Kupffer cells in high yield by means of an enterotoxin." in: Experimental cell research, Vol. 150, Issue 1, pp. 194-204, (1984) (PubMed).
M protein antibody, matrix protein antibody, M antibody
物质类
Viral Protein
背景
SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The complete sequence of the SARS virus release the coronavirus contains 25 open reading frames. SARS-m is a membrane (M) protein which plays a the key player in virion assembly. One of its functions is to mediate the incorporation of the spikes into the viral envelope.