IGF1R 抗体
Quick Overview for IGF1R 抗体 (ABIN966348)
抗原
See all IGF1R 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Crude ascites.
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免疫原
- Ni-NTA purified truncated recombinant IGF1R-His expressed in E. Coli strain BL21 (DE3)
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亚型
- IgG2b
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应用备注
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Western Blot: 1: 500- 1: 2,000
IHC(P): 1: 500- 1: 2,000
IHC(F): 1: 500- 1: 2,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test. -
限制
- 仅限研究用
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状态
- Liquid
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储存条件
- -20 °C
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Estradiol decreases IGF-1 and IGF-1 receptor expression in rat aortic smooth muscle cells. Mechanisms for its atheroprotective effects." in: The Journal of biological chemistry, Vol. 275, Issue 49, pp. 38921-8, (2001) (PubMed).
: "Physical mapping of the conjugative bacteriocin plasmid pPD1 of Enterococcus faecalis and identification of the determinant related to the pheromone response." in: Journal of bacteriology, Vol. 177, Issue 19, pp. 5574-81, (1995) (PubMed).
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Estradiol decreases IGF-1 and IGF-1 receptor expression in rat aortic smooth muscle cells. Mechanisms for its atheroprotective effects." in: The Journal of biological chemistry, Vol. 275, Issue 49, pp. 38921-8, (2001) (PubMed).
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- IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))
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别名
- IGF1R
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背景
- IGF1R(insulin-like growth factor 1 receptor), a transmembrane receptor tyrosine kinase, is widely expressed in many cell types within fetal and postnatal tissues, and in many cell lines. Upon binding to its ligands, IGF-I and IGF-II, receptor autophosphorylation occurs. The triple tyrosine cluster within the kinase domain (Tyr1131, Tyr1135 and Tyr1136) is the earliest major site of autophosphorylation. Phosphorylation of these three tyrosine residues is necessary for kinase activation.Insulin receptors (IRs) share significant similarity with IGF1 receptors in both structure and function,including an equivalent triple tyrosine cluster within the activation loop of the kinase domain (Tyr1146, Tyr1150 and Tyr1151).Tyrosine autophosphorylation of insulin receptor is one of the earliest cellular responses to insulin stimulation. Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151. Full kinase activation requires the triple tyrosine phosphorylation.
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途径
- RTK signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy
抗原
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