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NF-kB p65 抗体 (C-Term)

Name: NF-kB p65 抗体 (C-Term)
SKU: ABIN964680
Availability: OutOfStock

This anti-NF-kB p65 antibody is a 小鼠单克隆 antibody detecting NF-kB p65 in WB, IHC, ELISA, IF 和 FM. Suitable for 人. This Primary Antibody has been cited in 2+ publications.

anti NFKB p65 (Rel A) monoclonal antibody was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. (NF-kB p65 抗体 (C-Term))

Antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. (NF-kB p65 抗体 (C-Term))

IHC

Anti NFkB monoclonal antibody – Immunocytochemistry Tissue: Human Fibroblasts Top: Before activation Bottom: After activation with poly IC Left: 600-401-271 anti p65 NLS specific lot 18372 Right: Monoclonal antibody C-Term The two antibodies that are shown target different regions of the p65 protein. (NF-kB p65 抗体 (C-Term))

Immunofluorescence (IF) image for anti-Nuclear Factor-kB p65 (NFkBP65) (C-Term) antibody (ABIN964680) (NF-kB p65 抗体 (C-Term))

Monoclonal anti NFKB p65 (Rel A) antibody was used to detect p65 in b. (NF-kB p65 抗体 (C-Term))

monoclonal anti p65 was used to detect p65 by Western blot. (NF-kB p65 抗体 (C-Term))

6 images

(2 references)

产品编号 ABIN964680

Supplier Product No.: 200-301-065

发货至: 中国

Quick Overview for NF-kB p65 抗体 (C-Term) (ABIN964680)

抗原

See all NF-kB p65 (NFkBP65) 抗体

NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This NF-kB p65 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

克隆位点

27F9-G4

Discover our top product NFkBP65 Primary Antibody

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C-Term

Supplier Product No.

200-301-065

Supplier

Rockland

原理

NFkB p65 Antibody

交叉反应（详细）

Reactivity was confirmed by ELISA against peptide conjugated carrier protein and by Western blot against HeLa whole cell lysate.

产品特性

Synonyms: mouse anti-NF-kB p65 Antibody, mouse anti-Rel A antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, Nuclear factor NF-kappa-B p65 subunit

纯化方法

Anti-NF-kB p65 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography and showed a single band by IEP (immunoelectrophoresis) when tested with anti-mouse antibody.

过滤

Sterile filtered

免疫原

Immunogen: NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Conjugated Peptide

亚型

IgG2a kappa
anti-Nuclear Factor-kB p65 (NFkBP65) (AA 51-100) antibody
NFkBP65 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, 兔, 小鸡 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pSer536) antibody
NFkBP65 适用: 人, 小鼠, 大鼠, Cow, 小鸡 WB, ELISA, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) antibody
NFkBP65 适用: 人, 小鼠, 大鼠, 斑马鱼 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pSer536) antibody
NFkBP65 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (Center) antibody
NFkBP65 适用: 人, 小鼠 WB, IF, IHC (p), ICC, ChIP 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (AA 19-306) antibody
NFkBP65 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (acLys314), (acLys315) antibody
NFkBP65 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pThr435) antibody
NFkBP65 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (pSer281) antibody
NFkBP65 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor-kB p65 (NFkBP65) (AA 50-180) antibody
NFkBP65 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

Immunohistochemistry Dilution: 1:200-1:600
Application Note: Anti-NF-kB p65 Antibody is a mouse monoclonal antibody directed against NFkB p65 (Rel A) and recognizes a 65 kD band by Western blot against HeLa whole cell lysate. Control peptide (100-4165p) is sold separately. This product tested in WB, ICC, IHC, and IF.
Western Blot Dilution: 1:1,000-1:5,000
ELISA Dilution: 1:50,000-1:100,000
IF Microscopy Dilution: 1:5,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store NF-kB p65 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Sikorski, Mehta, Inngjerdingen, Thakor, Kling, Kalina, Nyman, Stensland, Zhou, de Souza, Holden, Stuchly, Templin, Lund-Johansen: "A high-throughput pipeline for validation of antibodies." in: Nature methods, Vol. 15, Issue 11, pp. 909-912, (2019) (PubMed).

Merkhofer, Cogswell, Baldwin: "Her2 activates NF-kappaB and induces invasion through the canonical pathway involving IKKalpha." in: Oncogene, Vol. 29, Issue 8, pp. 1238-48, (2010) (PubMed).
抗原

NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

别名

RELA

背景

Background: NFkappaB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkappaB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkappaBalpha. Cell Biology, Nuclear Signaling, Neuroscience and Signal Transduction Research.

基因ID

5970, 223468676

UniProt

Q04206

途径

NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins