CTCF 抗体 (C-Term)
(1 reference)
Quick Overview for CTCF 抗体 (C-Term) (ABIN964615)
抗原
See all CTCF 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-401-c42
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Supplier
- Rockland
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原理
- CTCF Antibody
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交叉反应 （详细）
- This antibody reacts with endogenous CTCF protein.
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产品特性
- Synonyms: rabbit anti-CTCF Antibody, Transcriptional repressor CTCF, 11-zinc finger protein, CCCTC-binding factor, CTCFL paralog
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纯化方法
- Anti-CTCF was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: CTCF affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of CTCF protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 1-260) antibody
CTCF 适用: 人 WB, IHC, IF, IP, ChIP 宿主: 兔 Polyclonal unconjugated
anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) antibodyCTCF 适用: 人 WB, ChIP-seq, CUT&RUN 宿主: 兔 Monoclonal unconjugated Recombinant Antibody
anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) antibodyCTCF 适用: 人 WB, IF, IP, ChIP 宿主: 兔 Monoclonal unconjugated
anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (C-Term) antibodyCTCF 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 1-260) antibodyCTCF 适用: 人 WB, IHC, ELISA, ChIP 宿主: 兔 Polyclonal unconjugated
anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 334-364) antibodyCTCF 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB53773 unconjugated
anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (N-Term) antibodyCTCF 适用: 人, 小鼠, 大鼠, Cow, 犬, 兔, 马, 猴, 豚鼠, Bat, 小鸡, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 521-727) antibodyCTCF 适用: 人, 小鼠, 大鼠 WB, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 451-550) antibodyCTCF 适用: 人 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 1-100) antibodyCTCF 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 5 μg/mL
Application Note: CTCF antibody has been tested by ELISA, Immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 82.8 kDa or 150 kDa in size corresponding to CTCF by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500-1:2:000
ELISA Dilution: 1:10,000-1:50,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.3 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store CTCF antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Domain-Specific and Stage-Intrinsic Changes in Tcrb Conformation during Thymocyte Development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 195, Issue 3, pp. 1262-72, (2015) (PubMed).
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: "Domain-Specific and Stage-Intrinsic Changes in Tcrb Conformation during Thymocyte Development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 195, Issue 3, pp. 1262-72, (2015) (PubMed).
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- CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))
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别名
- CTCF
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背景
- Background: Transcriptional repressor CTCF (also known as CCCTC-binding factor) is a transcription factor encoded by the CTCF gene. The CTCF, and the closely related Brother of the Regulator of Imprinted Sites (BORIS), are highly conserved zinc finger proteins implicated in diverse regulatory functions, including transcriptional activation/repression, insulation, imprinting, and X chromosome inactivation. Expression of BORIS is restricted to spermatocytes and is mutually exclusive of CTCF expression. CTCF is ubiquitously expressed in higher eukaryotes and contains a highly conserved and eleven zinc finger central DNA-binding domain, having very high homology between mouse, chicken, and human and is embedded within slightly divergent N and C termini. CTFC plays a critical role in the epigenetic regulation and chromatin remodeling. CTCF has been reported to bind to a variety of DNA target sites that perform distinct functions, including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting.
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基因ID
- 10664
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NCBI登录号
- NP_006556
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UniProt
- P49711
抗原
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