Western Blotting (WB), ELISA, Immunofluorescence (IF)
特异性
Human LTF / Lactoferrin. The reactivity of the antiserum is restricted to lactoferrin as tested in indirect binding immunoblotting and indirect immunoperoxidase staining. Cross-reactivity The antiserum does not react with any other component of the human milk or plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Usage: This product is intended for use to identify the presence of lactoferrin in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards, in ELISA from 1:50 upwards, in Western blotting from 1:250 upwards. These data should be interpreted as general recommendations only.
限制
仅限研究用
状态
Lyophilized
溶解方式
Sterile distilled water 0.5 ml
浓度
Lot specific
缓冲液
Lyophilized from PBS, pH 7.2
注意事项
Avoid repeat freeze-thaw cycles.
储存条件
4 °C,-20 °C
储存方法
Long term: -20°C Short term: +4°C. Avoid repeat freeze-thaw cycles.