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小鼠 anti-Cow Ig Light Chains Antibody

FACS, IHC (fro), WB Monoclonal IVA285-1 IgG1 unconjugated
产品编号 ABIN94401
发货至: 中国
  • 抗原
    Ig Light Chains
    适用
    Cow, 绵羊
    宿主
    • 2
    小鼠
    克隆类型
    • 2
    单克隆
    应用范围
    • 2
    • 2
    • 2
    • 2
    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
    特异性
    The antibody IVA285-1 reacts with bovine Ig (light chains). Ig light chains (kappa and lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
    交叉反应 (详细)
    Bovine, Sheep
    纯化方法
    Purified by protein-A affinity chromatography.
    纯度
    > 95 % (by SDS-PAGE)
    免疫原
    Fraction of Bovine immunoglobulin.
    克隆位点
    IVA285-1
    亚型
    IgG1
  • 应用备注
    Western blotting: Recommended dilution: 0,5-1 μg/mL, overnight at 4 °C. Sample preparation: bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12 % separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM.
    Flow cytometry: Recommended dilution: 1-4 μg/mL
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze.
    储存条件
    4 °C
    储存方法
    Store at 2-8°C. Do not freeze.
  • Antalíková, Simon, Jankovicová, Horovská, Dusinský, Hluchý: "Monoclonal antibody to the light chain of bovine immunoglobulin." in: Hybridoma (2005), Vol. 25, Issue 5, pp. 309-12, (2006) (PubMed).

  • 抗原
    Ig Light Chains
    背景
    Immunoglobulin light chains
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