HLAG 抗体
(1 reference)Quick Overview for HLAG 抗体 (ABIN94376)
抗原
See all HLAG 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-HLA-G Purified
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特异性
- The antibody MEM-G/11 recognizes an extracellular epitope of HLA-G1 antigen. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.
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交叉反应 （详细）
- Human
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
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亚型
- IgG1
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anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody
HLAG 适用: 人 WB, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal MEM-G-1 unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (PE)HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-G-9 PE
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibodyHLAG 适用: 人 ELISA, FACS, IP, IHC (fro), ICC 宿主: 小鼠 Monoclonal MEM-G-9 unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibodyHLAG 适用: 人 ELISA, FACS, IP, IHC (fro), ICC 宿主: 小鼠 Monoclonal MEM-G-9 unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (Biotin)HLAG 适用: 人 ELISA, FACS, IP, IHC (fro), ICC 宿主: 小鼠 Monoclonal MEM-G-9 Biotin
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (FITC)HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-G-9 FITC
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (APC)HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-G-9 APC
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (AA 62-89) antibodyHLAG 适用: 人 ELISA, FACS, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (Biotin)HLAG 适用: 人 WB, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal MEM-G-1 Biotin
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (C-Term) antibody (PE)HLAG 适用: 人 ICFC 宿主: 小鼠 Monoclonal 5A6G7 PE
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应用备注
- Flow cytometry: The antibody MEM-G/11 does not cross-block standard MEM-G/9 antibody. Recommended dilution: 2 μg/mL.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Disulfide bond-mediated dimerization of HLA-G on the cell surface." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 25, pp. 16180-5, (2002) (PubMed).
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: "Disulfide bond-mediated dimerization of HLA-G on the cell surface." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 25, pp. 16180-5, (2002) (PubMed).
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- HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
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别名
- HLA-G
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背景
- Major histocompatibility complex, class I, G,Human leukocyte antigen G (HLA-G), belonging to MHC class I glycoproteins, plays important roles in both physiological and pathological immunotolerance. It gives an inhibitory signal to cytotoxic T cells, NK cells, monocytes, and some other immune cells. It also induces regulatory T cells and anti-inflammatory macrophages. HLA-G is important e.g. for maternal tolerance to the fetus, and for immunomodulation in particular adult tissues, such as in cornea, pancreatic islets, thymus and other. On the other hand, it is expressed in many solid and hematologic malignancies, where it contributes to evasion of the immune surveillance. HLA-G expression pattern in cancer is an important prognostic factor regarding a poor clinical outcome. Unlike most other MHC glycoproteins, HLA-G acts as an immune checkpoint molecule rather than as an antigen presenting molecule. It concerns both transmembrane and soluble HLA-G isoforms. Among other, HLA-G can promote Th2 immunological response and downregulate Th1 immunological response. For its benefits regarding allograft tolerance, including embryo implantation, soluble HLA-G (sHLA-G) can be used as a marker of developmental potential of embryos during the process of in vitro fertilization. Similarly, sHLA-G concentrations in maternal serum are decreased in preeclampsia. Transplanted patients with increased sHLA-G serum levels have improved allograft acceptance. On the other hand, increased sHLA-G can also indicate presence of malignant (sometimes also of benign) tumor cells. Another important topic is induction of HLA-G expression (sometimes associated with shedding of HLA-G from the cell surface) by some anti-cancer or anti-viral therapies, which can weaken the therapy effect. Monitoring of HLA-G in patients thus has a wide usage.
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基因ID
- 3135
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UniProt
- P17693
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
抗原
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