HLAG 抗体 (FITC)
(6 references)Our Local Distributor
北京 101111
Quick Overview for HLAG 抗体 (FITC) (ABIN94361)
抗原
See all HLAG 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Anti-HLA-G FITC
-
无交叉反应
- 小鼠, 大鼠
-
交叉反应 （详细）
- Human
-
产品特性
- The antibody 01G recognizes membrane-bound form of HLA-G (full-length HLA-G1), but not soluble forms. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.
-
纯化方法
- Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
-
免疫原
- HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
-
亚型
- IgG1
-
-
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody
HLAG 适用: 人 WB, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal MEM-G-1 unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (PE)HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-G-9 PE
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibodyHLAG 适用: 人 ELISA, FACS, IP, IHC (fro), ICC 宿主: 小鼠 Monoclonal MEM-G-9 unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibodyHLAG 适用: 人 ELISA, FACS, IP, IHC (fro), ICC 宿主: 小鼠 Monoclonal MEM-G-9 unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (Biotin)HLAG 适用: 人 ELISA, FACS, IP, IHC (fro), ICC 宿主: 小鼠 Monoclonal MEM-G-9 Biotin
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (FITC)HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-G-9 FITC
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (APC)HLAG 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-G-9 APC
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (AA 62-89) antibodyHLAG 适用: 人 ELISA, FACS, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (Biotin)HLAG 适用: 人 WB, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal MEM-G-1 Biotin
anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (C-Term) antibody (PE)HLAG 适用: 人 ICFC 宿主: 小鼠 Monoclonal 5A6G7 PE
-
-
应用备注
- Flow cytometry: Recommended dilution: 5 μg/mL.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Do not freeze,Protect from prolonged exposure to light
-
储存条件
- 4 °C
-
储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
-
-
-
: "Expression of the immune-tolerogenic major histocompatibility molecule HLA-G in multiple sclerosis: implications for CNS immunity." in: Brain : a journal of neurology, Vol. 128, Issue Pt 11, pp. 2689-704, (2005) (PubMed).
: "Binding analysis of HLA-G specific antibodies to hematopoietic cells isolated from leukemia patients." in: Neoplasma, Vol. 50, Issue 5, pp. 331-8, (2003) (PubMed).
: "Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients." in: Leukemia research, Vol. 27, Issue 7, pp. 643-8, (2003) (PubMed).
: "Expression of the non-classical HLA-G antigen in tumor cell lines is extremely restricted." in: Neoplasma, Vol. 47, Issue 6, pp. 342-8, (2001) (PubMed).
: "Looking for HLA-G expression in human tumours." in: Journal of reproductive immunology, Vol. 43, Issue 2, pp. 263-73, (1999) (PubMed).
: "Expression of HLA G in human tumors is not a frequent event." in: International journal of cancer. Journal international du cancer, Vol. 81, Issue 4, pp. 512-8, (1999) (PubMed).
-
: "Expression of the immune-tolerogenic major histocompatibility molecule HLA-G in multiple sclerosis: implications for CNS immunity." in: Brain : a journal of neurology, Vol. 128, Issue Pt 11, pp. 2689-704, (2005) (PubMed).
-
- HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
-
别名
- HLA-G
-
背景
- Major histocompatibility complex, class I, G,Human leukocyte antigen G (HLA-G), belonging to MHC class I glycoproteins, plays important roles in both physiological and pathological immunotolerance. It gives an inhibitory signal to cytotoxic T cells, NK cells, monocytes, and some other immune cells. It also induces regulatory T cells and anti-inflammatory macrophages. HLA-G is important e.g. for maternal tolerance to the fetus, and for immunomodulation in particular adult tissues, such as in cornea, pancreatic islets, thymus and other. On the other hand, it is expressed in many solid and hematologic malignancies, where it contributes to evasion of the immune surveillance. HLA-G expression pattern in cancer is an important prognostic factor regarding a poor clinical outcome. Unlike most other MHC glycoproteins, HLA-G acts as an immune checkpoint molecule rather than as an antigen presenting molecule. It concerns both transmembrane and soluble HLA-G isoforms. Among other, HLA-G can promote Th2 immunological response and downregulate Th1 immunological response. For its benefits regarding allograft tolerance, including embryo implantation, soluble HLA-G (sHLA-G) can be used as a marker of developmental potential of embryos during the process of in vitro fertilization. Similarly, sHLA-G concentrations in maternal serum are decreased in preeclampsia. Transplanted patients with increased sHLA-G serum levels have improved allograft acceptance. On the other hand, increased sHLA-G can also indicate presence of malignant (sometimes also of benign) tumor cells. Another important topic is induction of HLA-G expression (sometimes associated with shedding of HLA-G from the cell surface) by some anti-cancer or anti-viral therapies, which can weaken the therapy effect. Monitoring of HLA-G in patients thus has a wide usage.
-
基因ID
- 3135
-
UniProt
- P17693
-
途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
抗原
-
