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Keratin 5/8 抗体

This anti-Keratin 5/8 antibody is a 小鼠 单克隆 antibody detecting Keratin 5/8 in WB, IHC (p), ICC 和 IP. Suitable for 人, 小鼠, Pig, 大鼠, 犬, Hamster, Cow 和 绵羊. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN94292
发货至: 中国

Quick Overview for Keratin 5/8 抗体 (ABIN94292)

抗原

Keratin 5/8 (KRT5/8)

适用

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人, 小鼠, Pig, 大鼠, 犬, Hamster, Cow, 绵羊

宿主

  • 23
小鼠

克隆类型

  • 23
单克隆

标记

  • 9
  • 4
  • 3
  • 1
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  • 1
  • 1
  • 1
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This Keratin 5/8 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

克隆位点

C-50
  • 原理

    Anti-Cytokeratin 5/8 Purified

    特异性

    The antibody C-50 reacts with cytokeratin peptides 5 and 8 (58 and 53 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins represented in epithelial tissues.

    无交叉反应

    小鸡, 非洲爪蟾

    交叉反应 (详细)

    Human, Sheep, Canine (Dog), Hamster, Mouse, Rat, Bovine, Porcine

    纯化方法

    Purified by protein-A affinity chromatography.

    纯度

    > 95 % (by SDS-PAGE)

    免疫原

    Cytoskeleton preparation from HeLa cells.

    亚型

    IgG1
  • 应用备注

    Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive control: human prostate.
    Western blotting: Recommended dilution: 1-2 μg/mL.

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Do not freeze.

    储存条件

    4 °C

    储存方法

    Store at 2-8°C. Do not freeze.
  • Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).

  • 抗原

    Keratin 5/8 (KRT5/8)

    别名

    Cytokeratin 5/8

    背景

    Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K5, K8, CK5, CK8, CYK5, CYK8, KRT5, KRT8
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