Keratin 5/8 抗体
-
- 抗原 See all Keratin 5/8 (KRT5/8) products
- Keratin 5/8 (KRT5/8)
-
适用
- 人, 小鼠, Pig, Cow, 大鼠, 犬, Hamster, 绵羊, 雪貂
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Keratin 5/8 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)
- 特异性
- It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
- 无交叉反应
- 小鸡, 非洲爪蟾
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Cytoskeletal preparation from HeLa cells
- 克隆位点
- C-50
- 亚型
- IgG1 kappa
-
anti-Keratin 5/8 (KRT5/8) antibody
KRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, 兔 WB, FACS, IHC, IHC (p), IHC (fro), ICC 宿主: 小鼠 Monoclonal RCK102 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, Cow, 大鼠, 犬, Hamster, 绵羊 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal C-50 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, 兔 WB, FACS, IHC, IHC (p), IHC (fro), ICC 宿主: 小鼠 Monoclonal RCK102 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, Cow, 大鼠, 犬, Hamster, 绵羊, 雪貂 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal SPM268 unconjugated
-
- 应用备注
-
Positive Control: HepG2 or A431 cells. Skin or Squamous cell carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
-
- 浓度
- 200 μg/mL
- 缓冲液
- 0.5 mL at 200 µg/mL of antibody Purified from Bioreactor Concentrate by Protein A/G chromatography. Supplied in 10 mM PBS, pH 7.4 with 0.05 % BSA and 0.05 % sodium azide. Contact us if you require this antibody in a different format.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 2 to 8°C. Antibody is stable for 24 months.
- 有效期
- 24 months
-
-
