Keratin 5/8 抗体
Our Local Distributor
北京 101111
Quick Overview for Keratin 5/8 抗体 (ABIN6941336)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Cytokeratin 5/8 Antibody
-
特异性
- It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
-
无交叉反应
- 小鸡, 非洲爪蟾
-
纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
-
免疫原
- Cytoskeletal preparation from HeLa cells
-
亚型
- IgG1, kappa
-
-
anti-Keratin 5/8 (KRT5/8) antibody
KRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, 兔 WB, FACS, IHC, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal RCK102 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, Cow, 绵羊 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal C-50 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, 兔 WB, FACS, IHC, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal RCK102 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, Cow, 绵羊, 雪貂 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal SPM268 unconjugated
-
-
应用备注
-
Positive Control: HepG2 or A431 cells. Skin or Squamous cell carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT)Optimal dilution for a specific application should be determined.
-
限制
- 仅限研究用
-
-
-
浓度
- 200 μg/mL
-
缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Store at 2 to 8°C. Antibody is stable for 24 months.
-
有效期
- 24 months
-
-
- Keratin 5/8 (KRT5/8)
-
别名
- KRT5 & KRT8
-
背景
-
CK5, DDD, EBS2, Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types, K5, Keratin complex 2, basic, Gene 5, Keratin Type II Cytoskeletal 5, Keratin-5, KRT5, KRT5A, Type II Cytoskeletal 5, Type-II Keratin Kb5. CK8, CARD2, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8
HepG2 or A431 cells. Skin or Squamous cell carcinoma.
-
分子量
- 58kDa (CK5), 52.5kDa (CK8)
-
基因ID
- 3852, 3856, 433845
-
UniProt
- P13647, P05787
抗原
-
