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Keratin 5/8 抗体 (AA 353-367) (Biotin)

KRT5/8 适用: 人 FACS, IHC, IHC (p), IHC (fro), ELISA 宿主: 小鼠 Monoclonal Biotin
产品编号 ABIN344035
发货至: 中国
  • 抗原 See all Keratin 5/8 (KRT5/8) products
    Keratin 5/8 (KRT5/8)
    抗原表位
    AA 353-367
    适用
    • 23
    • 19
    • 18
    • 15
    • 13
    • 13
    • 13
    • 10
    • 9
    • 5
    • 3
    • 2
    • 2
    宿主
    • 23
    小鼠
    克隆类型
    • 23
    单克隆
    标记
    • 11
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Keratin 5/8 antibody is conjugated to Biotin
    应用范围
    • 18
    • 15
    • 13
    • 13
    • 8
    • 8
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA
    特异性
    Reactivities on Cultured MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650. Cell lines (tested so far) SSC-12, bovine BMGE+H, BMGE-H, MDBK. Represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. Specifically reacts with cytokeratins 5 and 8 present in nearly all epithelia. Polypeptide reacting: Mr 52500, Mr 58000 cytokeratins (type II cytokeratins nos. 5+8) of human epithelial cells. Epitope has been mapped to aa353-367 on alpha helical rod domain. Species cross-reactivity: Human, bovine, murine, amphibia, porcine, hydra.
    纯化方法
    Protein A purified
    免疫原
    Human cytokeratin 8, purified from SDS PAGE.

    Type of Immunogen: Purified protein
    亚型
    IgG1
  • 应用备注
    Approved: ELISA, Flo, IHC, IHC-Fr, IHC-P

    Usage: Suitable for use in ELISA, FACS and Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material, Pretreatment With paraffin embedded sections, protease pretreatment is required prior to antibody application). Immunohistochemistry: 1:10-1:20, Incubation Time 1hr at RT, extended with paraffin sections (overnight at 2-8?C). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 浓度
    Lot specific
  • 抗原
    Keratin 5/8 (KRT5/8)
    别名
    Cytokeratin 5+8 (KRT5/8 产品)
    背景
    This antibody detects: Cytokeratin 5+8
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