Keratin 5/8 抗体
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北京 101111
Quick Overview for Keratin 5/8 抗体 (ABIN3025013)
抗原
适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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原理
- Cytokeratin 5/8 Antibody (azide and preservative free)
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无交叉反应
- 小鸡, 非洲爪蟾
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产品特性
- It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
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纯化方法
- Protein G affinity chromatography
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免疫原
- A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5/8 antibody.
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亚型
- IgG1, kappa
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anti-Keratin 5/8 (KRT5/8) antibody
KRT5/8 适用: 人, 小鼠, 大鼠, Pig, 犬, Hamster, 兔 WB, FACS, IHC, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal RCK102 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, 大鼠, Pig, 犬, Hamster, Cow, 绵羊 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal C-50 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, 大鼠, Pig, 犬, Hamster, Cow, 绵羊, 雪貂 WB, FACS, IHC, IF 宿主: 小鼠 Monoclonal C-50 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, 大鼠, Pig, 犬, Hamster, 兔 WB, FACS, IHC, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal RCK102 unconjugated
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应用备注
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Optimal dilution of the Cytokeratin 5/8 antibody should be determined by the researcher.
1. For staining of formalin-fixed tissues, digest sections with Trypsin at 1 mg/mL PBS, 15 min at RT
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Cytokeratin 5/8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Keratin 5/8 (KRT5/8)
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别名
- Cytokeratin 5/8
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背景
- It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
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UniProt
- P13647
抗原
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